Academic Journal
127 Plasma from Patients with Burn Injury Increases Endothelial Permeability In-Vitro
| العنوان: | 127 Plasma from Patients with Burn Injury Increases Endothelial Permeability In-Vitro |
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| المؤلفون: | Keyloun, John W, Carney, Bonnie C, Nisar, Saira, Moffatt, Lauren T, Shupp, Jeffrey W |
| المصدر: | Journal of Burn Care & Research ; volume 42, issue Supplement_1, page S86-S86 ; ISSN 1559-047X 1559-0488 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Rehabilitation, Emergency Medicine, Surgery |
| الوصف: | Introduction The contribution of endothelial injury to the pathogenesis of burn shock is not well characterized. Human umbilical endothelial cells (HUVECs) have been used to study endotheliopathy in myriad shock states. This work investigates the impact of burn patient plasma on the vascular endothelium and its barrier function. Methods HUVECs were seeded into the apical chambers of transwell plates and cultured over 5–7 days to a confluent monolayer which was confirmed by a transendothelial electrical resistance (TEER) of ≥30Ω. After IRB approval, plasma was collected from burn-injured patients 4 hours after admission. Demographic and injury characteristics were collected from the medical record. Plasma Syndecan-1 (SDC-1) was quantified by ELISA. HUVEC monolayers were exposed to 10% multi-donor pooled healthy human plasma (HHP) or burn patient plasma. Monolayers were subsequently incubated with FIT-C Dextran (40,000 kD). FIT-C diffusion through monolayers was measured in basal chamber supernatants. Monolayer permeability was measured with indices calculated by normalizing values to blank (transwell inserts) and HHP-treated monolayer FIT-C diffusion. HUVECs were also cultured on glass slides and exposed to HHP or burn patient plasma. Cells were fixed with 4% Paraformaldehyde and F-Actin was stained with Texas Red-Phalloidin. Intercellular gap area was calculated using imaging software. Differences between treatment conditions were analyzed with Welch’s t-test and one-way ANOVA, simple linear regression was used to characterize the relationship between plasma SDC-1 and permeability indices, significance was set at p < 0.05. Results Eight burn patient plasma samples were tested. Patients were mostly male (75%) with a mean age of 50±20 years and mean %TBSA burn of 37±34%. Five burn plasma samples significantly increased monolayer permeability. There were no significant differences between patient samples that increased permeability in age, TBSA, gender, or in-hospital mortality. Monolayer ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/jbcr/irab032.131 |
| الاتاحة: | http://dx.doi.org/10.1093/jbcr/irab032.131 http://academic.oup.com/jbcr/article-pdf/42/Supplement_1/S86/36837073/irab032.131.pdf |
| Rights: | https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
| رقم الانضمام: | edsbas.7FB71E75 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/jbcr/irab032.131 |
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