Academic Journal
ELK-1 ubiquitination status and transcriptional activity are modulated independently of F-Box protein FBXO25
| العنوان: | ELK-1 ubiquitination status and transcriptional activity are modulated independently of F-Box protein FBXO25 |
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| المؤلفون: | Quintero-Barceinas, Reyna Sara, Gehringer, Franziska, Ducker, Charles, Saxton, Janice, Shaw, Peter E. |
| بيانات النشر: | American Society for Biochemistry and Molecular Biology |
| سنة النشر: | 2021 |
| المجموعة: | University of Nottingham: Repository@Nottingham |
| مصطلحات موضوعية: | gene regulation, ETS transcription factor family, ubiquitin ligase, cell proliferation, development, ubiquitin-specific protease 17 (USP17) |
| الوصف: | The mitogen-responsive, ETS-domain transcription factor ELK-1 stimulates the expression of immediate early genes at the onset of the cell cycle and participates in early developmental programming. ELK-1 is subject to multiple levels of posttranslational control, including phosphorylation, SUMOylation, and ubiquitination. Recently, removal of monoubiquitin from the ELK-1 ETS domain by the Ubiquitin Specific Protease USP17 was shown to augment ELK-1 transcriptional activity and promote cell proliferation. Here we have used coimmunoprecipitation experiments, protein turnover and ubiquitination assays, RNA-interference and gene expression analyses to examine the possibility that USP17 acts antagonistically with the F-box protein FBXO25, an E3 ubiquitin ligase previously shown to promote ELK-1 ubiquitination and degradation. Our data confirm that FBXO25 and ELK-1 interact in HEK293T cells and that FBXO25 is active toward Hand1 and HAX1, two of its other candidate substrates. However, our data indicate that FBXO25 neither promotes ubiquitination of ELK-1 nor impacts on its transcriptional activity and suggest that an E3 ubiquitin ligase other than FBXO25 regulates ELK-1 ubiquitination and function. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 0021-9258 |
| Relation: | https://nottingham-repository.worktribe.com/output/5291909; Journal of Biological Chemistry; Volume 296; https://nottingham-repository.worktribe.com/file/5291909/1/1-s2.0-S0021925820002100-main |
| DOI: | 10.1074/jbc.ra120.014616 |
| الاتاحة: | https://doi.org/10.1074/jbc.ra120.014616 https://nottingham-repository.worktribe.com/file/5291909/1/1-s2.0-S0021925820002100-main https://nottingham-repository.worktribe.com/output/5291909 |
| Rights: | openAccess ; http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.7F8D2881 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00219258 |
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| DOI: | 10.1074/jbc.ra120.014616 |