Report
A Transposon-Based Genetic Screen in Mice Identifies Genes Altered in Colorectal Cancer
| العنوان: | A Transposon-Based Genetic Screen in Mice Identifies Genes Altered in Colorectal Cancer |
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| المؤلفون: | Starr, T. K., Allaei, R., Silverstein, K. A. T., Staggs, R. A., Sarver, A. L., Bergemann, T. L., Gupta, M., O'Sullivan, M. G., Matise, I., Dupuy, A. J., Collier, L. S., Powers, S., Oberg, A. L., Asmann, Y. W., Thibodeau, S. N., Tessarollo, L., Copeland, N. G., Jenkins, N. A., Cormier, R. T., Largaespada, D. A. |
| سنة النشر: | 2009 |
| المجموعة: | Cold Spring Harbor Laboratory: CSHL Institutional Repository |
| مصطلحات موضوعية: | bioinformatics, cancer, DNA, RNA structure, function, modification, diseases & disorders, genetics & nucleic acid processing, genomics and proteomics, animal, colon cancer, genes, structure and function, mouse, transposons, cancer types |
| الوصف: | Human colorectal cancers (CRCs) display a large number of genetic and epigenetic alterations, some of which are causally involved in tumorigenesis (drivers) and others that have little functional impact (passengers). To help distinguish between these two classes of alterations, we used a transposon-based genetic screen in mice to identify candidate genes for CRC. Mice harboring mutagenic Sleeping Beauty (SB) transposons were crossed with mice expressing SB transposase in gastrointestinal tract epithelium. Most of the offspring developed intestinal lesions, including intraepithelial neoplasia, adenomas, and adenocarcinomas. Analysis of over 16,000 transposon insertions identified 77 candidate CRC genes, 60 of which are mutated and/or dysregulated in human CRC and thus are most likely to drive tumorigenesis. These genes include APC, PTEN, and SMAD4. The screen also identified 17 candidate genes that had not previously been implicated in CRC, including POLI, PTPRK, and RSPO2. |
| نوع الوثيقة: | report |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://repository.cshl.edu/id/eprint/27473/1/Powers_Science_2009.pdf; Starr, T. K., Allaei, R., Silverstein, K. A. T., Staggs, R. A., Sarver, A. L., Bergemann, T. L., Gupta, M., O'Sullivan, M. G., Matise, I., Dupuy, A. J., Collier, L. S., Powers, S., Oberg, A. L., Asmann, Y. W., Thibodeau, S. N., Tessarollo, L., Copeland, N. G., Jenkins, N. A., Cormier, R. T., Largaespada, D. A. (March 2009) A Transposon-Based Genetic Screen in Mice Identifies Genes Altered in Colorectal Cancer. Science, 323 (5922). pp. 1747-1750. ISSN 0036-8075 |
| DOI: | 10.1126/science.1163040 |
| الاتاحة: | http://repository.cshl.edu/id/eprint/27473/ http://repository.cshl.edu/id/eprint/27473/1/Powers_Science_2009.pdf http://www.ncbi.nlm.nih.gov/pubmed/19251594 https://doi.org/10.1126/science.1163040 |
| رقم الانضمام: | edsbas.7F3EA837 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1126/science.1163040 |
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