Academic Journal
Regulatory T‐cell development and function are impaired in mice lacking membrane expression of full length intercellular adhesion molecule‐1
| العنوان: | Regulatory T‐cell development and function are impaired in mice lacking membrane expression of full length intercellular adhesion molecule‐1 |
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| المؤلفون: | Gottrand, Gaëlle, Courau, Tristan, Thomas‐Vaslin, Véronique, Prevel, Nicolas, Vazquez, Thomas, Ruocco, Maria Grazia, Lambrecht, Benedicte, Bellier, Bertrand, Colombo, Bruno M., Klatzmann, David |
| المساهمون: | Institut National Du Cancer |
| المصدر: | Immunology ; volume 146, issue 4, page 657-670 ; ISSN 0019-2805 1365-2567 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2015 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Summary To further investigate the contribution of intercellular adhesion molecule‐1 ( ICAM ‐1) to adaptive immune responses, we analysed T‐cell development and function in mice lacking full‐length ICAM ‐1 ( ICAM ‐1 tm1Jcgr ). Compared with wild‐type ( ICAM ‐1 WT ) mice, ICAM ‐1 tm1Jcgr mice have impaired thymocyte development. Proportions and numbers of double negative, double positive, mature CD 4 + and CD 8 + thymocytes, as well as of regulatory T (Treg) cells were also significantly decreased. In the periphery, ICAM ‐1 tm1Jcgr mice had significantly decreased proportions and numbers of naive and activated/memory CD 4 + and CD 8 + T cells, as well as of Treg cells, in lymph nodes but not in the spleen. In vitro activation of CD 4 + and CD 8 + T cells from ICAM ‐1 tm1Jcgr mice with anti‐ CD 3 antibodies and antigen‐presenting cells ( APC s) resulted in a significantly weaker proliferation, whereas proliferation induced with anti‐ CD 3 and anti‐ CD 28 antibody‐coated beads was normal. In vivo immunization of ICAM ‐1 tm1Jcgr mice resulted in normal generation of specific effector and memory immune responses that protect against a viral challenge. However, contrary to ICAM ‐1 WT mice, immunization‐induced specific effectors could not eradicate immunogen‐expressing tumours. Treg cells from ICAM ‐1 tm1Jcgr mice have abnormal activation and proliferation induced by anti‐ CD 3 antibody and APC s, and have markedly decreased suppressive activity in vitro . In contrast to ICAM ‐1 WT mice, they were unable to control experimentally induced colitis in vivo . Hence, our results further highlight the pleiotropic role of ICAM ‐1 in T‐cell‐dependent immune responses, with a major role in Treg cell development and suppressive function. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/imm.12533 |
| الاتاحة: | http://dx.doi.org/10.1111/imm.12533 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fimm.12533 https://onlinelibrary.wiley.com/doi/pdf/10.1111/imm.12533 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.7F0717D9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/imm.12533 |
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