Academic Journal
Involvement of Small Non-Coding RNA and Cell Antigens in Pathogenesis of Extramedullary Multiple Myeloma
| العنوان: | Involvement of Small Non-Coding RNA and Cell Antigens in Pathogenesis of Extramedullary Multiple Myeloma |
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| المؤلفون: | Vlachová Monika, Gregorová Jana, Vychytilová Petra, Gablo Natalia Anna, Radová Lenka, Pospíšilová Lenka, Almáši Martina, Štork Martin, Knechtová Zdenka, Minařík Jiří, Popková Tereza, Jelínek Tomáš, Hájek Roman, Pour Luděk, Říhová Lucie, Ševčíková Sabina |
| المصدر: | International Journal of Molecular Sciences |
| بيانات النشر: | MDPI |
| سنة النشر: | 2022 |
| المجموعة: | Masaryk University: Open Services of Information System / Masarykova univerzita: Veřejné služby Informačního systému |
| مصطلحات موضوعية: | multiple myeloma, NGS, microRNA, immunophenotyping, bioinformatics |
| الوصف: | Extramedullary multiple myeloma (EMD) is an aggressive disease; malignant plasma cells lose their dependence in the bone marrow microenvironment and migrate into tissues. EMD is a negative prognostic factor of survival. Using flow cytometry and next-generation sequencing, we aimed to identify antigens and microRNAs (miRNAs) involved in EMD pathogenesis. Flow cytometry analysis revealed significant differences in the level of clonal plasma cells between MM and EMD patients, while the expression of CD markers was comparable between these two groups. Further, miR-26a-5p and miR-30e-5p were found to be significantly down-regulated in EMD compared to MM. Based on the expression of miR-26a-5p, we were able to distinguish these two groups of patients with high sensitivity and specificity. In addition, the involvement of deregulated miRNAs in cell cycle regulation, ubiquitin-mediated proteolysis and signaling pathways associated with infections or neurological disorders was observed using GO and KEGG pathways enrichment analysis. Subsequently, a correlation between the expression of analyzed miRNAs and the levels of CD molecules was observed. Finally, clinicopathological characteristics as well as CD antigens associated with the prognosis of MM and EMD patients were identified. Altogether, we identified several molecules possibly involved in the transformation of MM into EMD. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://is.muni.cz/publication/2235241 |
| DOI: | 10.3390/ijms232314765 |
| الاتاحة: | https://is.muni.cz/publication/2235241 https://doi.org/10.3390/ijms232314765 |
| Rights: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.7EF280F1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/ijms232314765 |
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