التفاصيل البيبلوغرافية
| العنوان: |
Genomic dissection of Klebsiella pneumoniae infections in hospital patients reveals insights into an opportunistic pathogen |
| المؤلفون: |
Gorrie, CL, Mirceta, M, Wick, RR, Judd, LM, Lam, MMC, Gomi, R, Abbott, IJ, Thomson, NR, Strugnell, RA, Pratt, NF, Garlick, JS, Watson, KM, Hunter, PC, Pilcher, DV, McGloughlin, SA, Spelman, DW, Wyres, KL, Jenney, AWJ, Holt, KE |
| بيانات النشر: |
NATURE PORTFOLIO |
| سنة النشر: |
2022 |
| المجموعة: |
The University of Melbourne: Digital Repository |
| الوصف: |
Klebsiella pneumoniae is a major cause of opportunistic healthcare-associated infections, which are increasingly complicated by the presence of extended-spectrum beta-lactamases (ESBLs) and carbapenem resistance. We conducted a year-long prospective surveillance study of K. pneumoniae clinical isolates in hospital patients. Whole-genome sequence (WGS) data reveals a diverse pathogen population, including other species within the K. pneumoniae species complex (18%). Several infections were caused by K. variicola/K. pneumoniae hybrids, one of which shows evidence of nosocomial transmission. A wide range of antimicrobial resistance (AMR) phenotypes are observed, and diverse genetic mechanisms identified (mainly plasmid-borne genes). ESBLs are correlated with presence of other acquired AMR genes (median n = 10). Bacterial genomic features associated with nosocomial onset are ESBLs (OR 2.34, p = 0.015) and rhamnose-positive capsules (OR 3.12, p < 0.001). Virulence plasmid-encoded features (aerobactin, hypermucoidy) are observed at low-prevalence (<3%), mostly in community-onset cases. WGS-confirmed nosocomial transmission is implicated in just 10% of cases, but strongly associated with ESBLs (OR 21, p < 1 × 10-11). We estimate 28% risk of onward nosocomial transmission for ESBL-positive strains vs 1.7% for ESBL-negative strains. These data indicate that K. pneumoniae infections in hospitalised patients are due largely to opportunistic infections with diverse strains, with an additional burden from nosocomially-transmitted AMR strains and community-acquired hypervirulent strains. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| تدمد: |
2041-1723 |
| Relation: |
NHMRC/1061409; NHMRC/1043822; pii: 10.1038/s41467-022-30717-6; Gorrie, C. L., Mirceta, M., Wick, R. R., Judd, L. M., Lam, M. M. C., Gomi, R., Abbott, I. J., Thomson, N. R., Strugnell, R. A., Pratt, N. F., Garlick, J. S., Watson, K. M., Hunter, P. C., Pilcher, D. V., McGloughlin, S. A., Spelman, D. W., Wyres, K. L., Jenney, A. W. J. & Holt, K. E. (2022). Genomic dissection of Klebsiella pneumoniae infections in hospital patients reveals insights into an opportunistic pathogen. NATURE COMMUNICATIONS, 13 (1), https://doi.org/10.1038/s41467-022-30717-6.; http://hdl.handle.net/11343/316136 |
| الاتاحة: |
http://hdl.handle.net/11343/316136 |
| Rights: |
CC BY ; https://creativecommons.org/licenses/by/4.0 |
| رقم الانضمام: |
edsbas.7EBB3E41 |
| قاعدة البيانات: |
BASE |
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