Academic Journal
Molecular Characterization of the Dot/Icm-Translocated AnkH and AnkJ Eukaryotic-Like Effectors of
| العنوان: | Molecular Characterization of the Dot/Icm-Translocated AnkH and AnkJ Eukaryotic-Like Effectors of |
|---|---|
| المؤلفون: | Habyarimana, Fabien, Price, Chris T., Šantić, Marina, Al-Khodor, Souhaila, Kwaik, Yousef Abu |
| المصدر: | https://journals.asm.org/doi/10.1128/IAI.00913-09 ; Infection and Immunity ; Volume 78 ; Issue 3 ; ISSN 0019-9567 (Print) ; ISSN 1098-5522 (Online. |
| سنة النشر: | 2010 |
| المجموعة: | Repository of the University of Rijeka |
| مصطلحات موضوعية: | BIOMEDICINA I ZDRAVSTVO. Kliničke medicinske znanosti. Medicinska mikrobiologija, BIOMEDICINE AND HEALTHCARE. Clinical Medical Sciences. Medical Microbiology |
| الوصف: | Although most Dot/Icm-translocated effectors of are not required for intracellular proliferation, the eukaryotic-like ankyrin effectors, AnkH and AnkJ are required for intracellular proliferation. In this report, we show that the IcmSW chaperones are essential for translocation of AnkJ but not AnkH. The 10 C-terminal residues and the ANK domains of AnkH and AnkJ are required for translocation. Our data indicate that the two ANK domains of AnkH are critical domains required for the function of the effector in intracellular replication of . The and mutants are severely defective in intrapulmonary proliferation in mice. Expression of AnkH and AnkJ fusions within HEK293 cells show a punctuate distribution in the cytosol but no association with endocytic vesicles, the Golgi apparatus or the endoplasmic reticulum. Interestingly, the defect in intracellular proliferation of the or mutants is rescued in HEK293 cells expressing the respective protein. We conclude that AnkH and AnkJ are effectors translocated by the Dot/Icm system by distinct mechanisms and modulate distinct cytosolic processes in the host cell. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | Sveučilište u Rijeci. Medicinski fakultet. Katedra za mikrobiologiju i parazitologiju.; University of Rijeka. Faculty of Medicine. Department of Microbiology and Parasitology.; https://www.unirepository.svkri.uniri.hr/islandora/object/medri:4930; https://urn.nsk.hr/urn:nbn:hr:184:239208; https://www.unirepository.svkri.uniri.hr/islandora/object/medri:4930/datastream/FILE0 |
| الاتاحة: | https://www.unirepository.svkri.uniri.hr/islandora/object/medri:4930 https://urn.nsk.hr/urn:nbn:hr:184:239208 https://www.unirepository.svkri.uniri.hr/islandora/object/medri:4930/datastream/FILE0 |
| Rights: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.7E7D25BE |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |