Academic Journal
Targeting glycolysis in Th2 cells by pterostilbene attenuates clinical severities in an asthmatic mouse model and IL-4 production in peripheral blood from asthmatic patients
| العنوان: | Targeting glycolysis in Th2 cells by pterostilbene attenuates clinical severities in an asthmatic mouse model and IL-4 production in peripheral blood from asthmatic patients |
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| المؤلفون: | 劉傳騰, Liu, Chuan-Teng, 宋瑛琪, Song, Ying-Chyi, 吳采真, Shi, Ko-Chieh, Shiung, Ko-Chieh, Chen, I-Hsuan, 張東, Chang, Tung-Ti, 梁信杰, Liang, Shinn-Jye, 顏宏融 |
| المساهمون: | 醫學暨健康學院醫學檢驗暨生物技術學系 |
| بيانات النشر: | 亞洲大學 |
| سنة النشر: | 2022 |
| المجموعة: | ASIA University, Taiwan: Instiutional Repository (ASIAIR) / 歡迎光臨亞洲大學全球資訊網 |
| مصطلحات موضوعية: | asthma, glycolysis, house dust mite, pterostilbene, type 2 helper T cells |
| الوصف: | Asthma, a major non-communicable disease, affects both adults and children and is associated with high morbidity compared with other chronic diseases. The glycolysis-associated activation of type 2 helper T (Th2) cells is the critical immunopathological mechanism involved in asthma deterioration. Long-term use of steroids as a medical treatment for asthma induces side effects and resistance. Pterostilbene (PS), a stilbenoid compound found in blueberry and vines, exhibits antihyperglycemic and anti-inflammatory properties. Thus, we hypothesized that the modulation of T cell immunity by PS may be an applicable intervention to treat asthma. Airway hyperresponsiveness, interleukin (IL)-4 and IL-13 levels, IgE, IgG, pulmonary infiltrated monocytes and eosinophils, and mucosubstances were measured in house dust mite (HDM)-induced asthmatic mice under PS treatment. Bioenergetic metabolism, PI3K-mTOR signalling, GATA3 expression and histone acetylation in PS-treated Th2 cells were investigated. PS improved HDM-induced pulmonary allergic airway inflammation by inhibiting Th2 cell and eosinophil accumulation in HDM asthmatic mice both in the preventive and therapeutic models. Targeting glycolysis resulted in IL-4 inhibition via the downregulation of mTOR, GATA3 and histone acetylation in PS-treated Th2 cells. Glucose supplementation reversed the inhibitory effect of PS on Th2 cells in vitro. Adoptive transfer with glucose-treated Th2 cells enhanced Th2 activation and eosinophilic accumulation in PS-treated asthmatic mice. Furthermore, PS significantly inhibited IL-4 production of CD4+ T cells from the peripheral blood mononuclear cells of patients with asthma. PS attenuates HDM-induced asthma via the inhibition of the Glut1/mTOR/GATA3 axis in Th2 cells, which supports the potential pharmaceutical application of PS treatment for asthma. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 81 bytes; text/html |
| اللغة: | English |
| Relation: | http://asiair.asia.edu.tw/ir/handle/310904400/115461; http://asiair.asia.edu.tw/ir/bitstream/310904400/115461/1/index.html |
| الاتاحة: | http://asiair.asia.edu.tw/ir/handle/310904400/115461 http://asiair.asia.edu.tw/ir/bitstream/310904400/115461/1/index.html |
| رقم الانضمام: | edsbas.7E565C58 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |