Academic Journal
Case-Control Study of Individuals with Discrepant Nucleocapsid and Spike Protein SARS-CoV-2 IgG Results
| العنوان: | Case-Control Study of Individuals with Discrepant Nucleocapsid and Spike Protein SARS-CoV-2 IgG Results |
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| المؤلفون: | Wang, Hannah, Wiredja, Danica, Yang, Lu, Bulterys, Philip L, Costales, Cristina, Röltgen, Katharina, Manalac, Justin, Yee, Jennifer, Zehnder, James, Shi, Run Zhang, Boyd, Scott D, Pinsky, Benjamin A |
| المصدر: | Clinical Chemistry ; ISSN 0009-9147 1530-8561 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2021 |
| الوصف: | Background Laboratory-based methods for SARS-CoV-2 antibody detection vary widely in performance. However, there are limited prospectively-collected data on assay performance, and minimal clinical information to guide interpretation of discrepant results. Methods Over a 2-week period, 1080 consecutive plasma samples submitted for clinical SARS-CoV-2 IgG testing were tested in parallel for anti-nucleocapsid IgG (anti-N, Abbott) and anti-spike IgG (anti-S1, EUROIMMUN). Chart review was conducted for samples testing positive or borderline on either assay, and for an age/sex-matched cohort of samples negative by both assays. CDC surveillance case definitions were used to determine clinical sensitivity/specificity and conduct receiver operating characteristics curve analysis. Results There were 52 samples positive by both methods, 2 positive for anti-N only, 34 positive for anti-S1 only, and 27 borderline for anti-S1. Of the 34 individuals positive for anti-S1 alone, 8 (24%) had confirmed COVID-19. No anti-S1 borderline cases were positive for anti-N or had confirmed/probable COVID-19. The anti-N assay was less sensitive (84.2% [95% CI 72.1-92.5%] vs 94.7% [95% CI 85.4-98.9%]) but more specific (99.2% [95% CI 95.5-100%] vs 86.9% [95% CI 79.6-92.3%]) than anti-S1. Abbott anti-N sensitivity could be improved to 96.5% with minimal effect on specificity if the index threshold was lowered from 1.4 to 0.6. Conclusion Real-world concordance between different serologic assays may be lower than previously described in retrospective studies. These findings have implications for the interpretation of SARS-CoV-2 IgG results, especially with the advent of spike antigen-targeted vaccination, as a subset of patients with true infection are anti-N negative and anti-S1 positive. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/clinchem/hvab045 |
| DOI: | 10.1093/clinchem/hvab045/36600785/hvab045.pdf |
| DOI: | 10.1093/clinchem/hvab045/37940700/hvab045.pdf |
| الاتاحة: | http://dx.doi.org/10.1093/clinchem/hvab045 http://academic.oup.com/clinchem/advance-article-pdf/doi/10.1093/clinchem/hvab045/36600785/hvab045.pdf http://academic.oup.com/clinchem/advance-article-pdf/doi/10.1093/clinchem/hvab045/37940700/hvab045.pdf |
| Rights: | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: | edsbas.7E3C0014 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/clinchem/hvab045 |
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