Academic Journal
Increased mitochondrial protein levels and bioenergetics in the musculus rectus femoris of Wfs1-deficient mice
| العنوان: | Increased mitochondrial protein levels and bioenergetics in the musculus rectus femoris of Wfs1-deficient mice |
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| المؤلفون: | Eimre, M., Paju, K., Peet, N., Kadaja, L., Tarrend, M., Kasvandik, S., Seppet, J., Ivask, M., Orlova, E., Kõks, S. |
| بيانات النشر: | Hindawi Publishing |
| سنة النشر: | 2018 |
| الوصف: | Wfs1 deficiency leads to a progressive loss of plasma insulin concentration, which should reduce the consumption of glucose in insulin-dependent tissues, causing a variety of changes in intracellular energy metabolism. Our objective here was to assess the changes in the amount and function of mitochondrial proteins in different muscles of Wfs1-deficient mice. Mitochondrial functions were assayed by high-resolution oxygraphy of permeabilized muscle fibers; the protein amount was evaluated by liquid chromatography tandem mass spectrometry (LC/MS/MS) analysis and mRNA levels of the uncoupler proteins UCP2 and UCP3 by real-time PCR; and citrate synthase (CS) activity was determined spectrophotometrically in muscle homogenates. Compared to controls, there were no changes in proton leak and citrate synthase activity in the heart and m. soleus tissues of Wfs1-deficient mice, but significantly higher levels of both of these factors were observed in the m. rectus femoris; mitochondrial proteins and mRNA of UCP2 were also higher in the m. rectus femoris. ADP-stimulated state 3 respiration was lower in the m. soleus, remained unchanged in the heart, and was higher in the m. rectus femoris. The mitochondrial protein amount and activity are higher in Wfs1-deficient mice, as are mitochondrial proton leak and oxygen consumption in m. rectus femoris. These changes in muscle metabolism may be important for identifying the mechanisms responsible for Wolfram syndrome and diabetes. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | |
| اللغة: | English |
| تدمد: | 1942-0900 |
| Relation: | ispartof: Oxidative Medicine and Cellular Longevity spage 1 epage 12 vol 2018; WOS:000451858700001; https://doi.org/10.1155/2018/3175313; 991005540564807891; https://researchportal.murdoch.edu.au/esploro/outputs/journalArticle/Increased-mitochondrial-protein-levels-and-bioenergetics/991005540564807891; https://researchportal.murdoch.edu.au/view/delivery/61MUN_INST/12135173480007891/13136860080007891; alma:61MUN_INST/bibs/991005540564807891 |
| DOI: | 10.1155/2018/3175313 |
| الاتاحة: | https://doi.org/10.1155/2018/3175313 https://researchportal.murdoch.edu.au/esploro/outputs/journalArticle/Increased-mitochondrial-protein-levels-and-bioenergetics/991005540564807891 https://researchportal.murdoch.edu.au/view/delivery/61MUN_INST/12135173480007891/13136860080007891 |
| Rights: | © 2018 Margus Eimre et al. ; Open |
| رقم الانضمام: | edsbas.7E173DAB |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19420900 |
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| DOI: | 10.1155/2018/3175313 |