Academic Journal
Hydroxylated polymethoxyflavones reduce the activity of pancreatic lipase, inhibit adipogenesis and enhance lipolysis in 3T3-L1 mouse embryonic fibroblast cells
| العنوان: | Hydroxylated polymethoxyflavones reduce the activity of pancreatic lipase, inhibit adipogenesis and enhance lipolysis in 3T3-L1 mouse embryonic fibroblast cells |
|---|---|
| المؤلفون: | Ahmad, Bilal, Friar, Emily P., Vohra, Muhammad Sufyan, Khan, Nasar, Serpell, Christopher J., Garrett, Michelle D., Loo, Jason Siau Ee, Fong, Isabel Lim, Wong, Eng Hwa |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2023 |
| المجموعة: | University of Kent: KAR - Kent Academic Repository |
| مصطلحات موضوعية: | Q Science, QD Chemistry |
| الوصف: | Hydroxylated polymethoxyflavones (HPMFs) have been shown to possess various anti-disease effects, including against obesity. This study investigates the anti-obesity effects of HPMFs in further detail, aiming to gain understanding of their mechanism of action in this context. The current study demonstrates that two HPMFs; 3′-hydroxy-5,7,4′,5′-tetramethoxyflavone (3′OH-TetMF) and 4′-hydroxy-5,7,3′,5′-tetramethoxyflavone (4′OH-TetMF) possess anti-obesity effects. They both significantly reduced pancreatic lipase activity in a competitive manner as demonstrated by molecular docking and kinetic studies. In cell studies, it was revealed that both of the HPMFs suppress differentiation of 3T3-L1 mouse embryonic fibroblast cells during the early stages of adipogenesis. They also reduced expression of key adipogenic and lipogenic marker genes, namely peroxisome proliferator-activated receptor-gamma (PPARγ), CCAAT/enhancer-binding protein α and β (C/EBP α and β), adipocyte binding protein 2 (aP2), fatty acid synthase (FASN), and sterol regulatory element-binding protein 1 (SREBF 1). They also enhanced the expression of cell cycle genes, i.e., cyclin D1 (CCND1) and C-Myc, and reduced cyclin A2 expression. When further investigated, it was also observed that these HPMFs accelerate lipid breakdown (lipolysis) and enhance lipolytic genes expression. Moreover, they also reduced the secretion of proteins (adipokines), including pro-inflammatory cytokines, from mature adipocytes. Taken together, this study concludes that these HPMFs have anti-obesity effects, which are worthy of further investigation. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://kar.kent.ac.uk/101195/1/E.Friar%20-%20Hydroxylated%20polymethoxyflavones%20reduce%20the%20activity%20of%20pancreatic%20lipase%20-%20PPDF.pdf; Ahmad, Bilal, Friar, Emily P., Vohra, Muhammad Sufyan, Khan, Nasar, Serpell, Christopher J., Garrett, Michelle D., Loo, Jason Siau Ee, Fong, Isabel Lim, Wong, Eng Hwa (2023) Hydroxylated polymethoxyflavones reduce the activity of pancreatic lipase, inhibit adipogenesis and enhance lipolysis in 3T3-L1 mouse embryonic fibroblast cells. Chemico-Biological Interactions, 379 . Article Number 110503. ISSN 0009-2797. (doi:10.1016/j.cbi.2023.110503 ) (KAR id:101195 ) |
| DOI: | 10.1016/j.cbi.2023.110503 |
| الاتاحة: | https://kar.kent.ac.uk/101195/ https://doi.org/10.1016/j.cbi.2023.110503 |
| Rights: | cc_by |
| رقم الانضمام: | edsbas.7DB6F730 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.cbi.2023.110503 |
|---|