التفاصيل البيبلوغرافية
| العنوان: |
The Pathophysiologic Role of Gelsolin in Chronic Kidney Disease: Focus on Podocytes |
| المؤلفون: |
Chia-Jung Yu, Dian W. Damaiyanti, Shian-Jang Yan, Chih-Hsing Wu, Ming-Jer Tang, Dar-Bin Shieh, Peter P. Liu, Ping-Yen Liu |
| المصدر: |
International Journal of Molecular Sciences; Volume 22; Issue 24; Pages: 13281 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2021 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
podocyte, gelsolin, actin, chronic kidney disease |
| جغرافية الموضوع: |
agris |
| الوصف: |
Chronic kidney disease (CKD) is normally related to proteinuria, a common finding in a compromised glomerular filtration barrier (GFB). GFB is a structure composed of glomerular endothelial cells, the basement membrane, and the podocytes. CKD with podocyte damage may be associated with actin cytoskeleton reorganization, resulting in podocyte effacement. Gelsolin plays a critical role in several diseases, including cardiovascular diseases and cancer. Our current study aimed to determine the connection between gelsolin and podocyte, and thus the mechanism underlying podocyte injury in CKD. Experiments were carried out on Drosophila to demonstrate whether gelsolin had a physiological role in maintaining podocyte. Furthermore, the survival rate of gelsolin-knocked down Drosophila larvae was extensively reduced after AgNO3 exposure. Secondly, the in vitro podocytes treated with puromycin aminonucleoside (PAN) enhanced the gelsolin protein expression, as well as small GTPase RhoA and Rac1, which also regulated actin dynamic expression incrementally with the PAN concentrations. Thirdly, we further demonstrated in vivo that GSN was highly expressed inside the glomeruli with mitochondrial dysfunction in a CKD mouse model. Our findings suggest that an excess of gelsolin may contribute to podocytes damage in glomeruli. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Molecular Pathology, Diagnostics, and Therapeutics; https://dx.doi.org/10.3390/ijms222413281 |
| DOI: |
10.3390/ijms222413281 |
| الاتاحة: |
https://doi.org/10.3390/ijms222413281 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.7DB58B28 |
| قاعدة البيانات: |
BASE |