Academic Journal
Overexpression of Ras-Related C3 Botulinum Toxin Substrate 2 Radiosensitizes Melanoma Cells In Vitro and In Vivo
| العنوان: | Overexpression of Ras-Related C3 Botulinum Toxin Substrate 2 Radiosensitizes Melanoma Cells In Vitro and In Vivo |
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| المؤلفون: | Wentao Hu, Lin Zhu, Weiwei Pei, Shuxian Pan, Ziyang Guo, Anqing Wu, Hailong Pei, Jing Nie, Bingyan Li, Yoshiya Furusawa, Teruaki Konishi, Tom K. Hei, Guangming Zhou |
| المصدر: | Oxidative Medicine and Cellular Longevity, Vol 2019 (2019) |
| بيانات النشر: | Hindawi Limited |
| سنة النشر: | 2019 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Cytology, QH573-671 |
| الوصف: | Radioresistance is the major obstacle in the radiotherapy of the malignant melanoma. Thus, it is of importance to increase the radiosensitivity of melanoma cells. In the present study, the radioresistant melanoma cell line OCM-1 with inducible overexpression of Ras-related C3 botulinum toxin substrate 2 was established based on a radiation-inducible early growth response gene (Egr-1) promoter. The effects of Ras-related C3 botulinum toxin substrate 2 overexpression on the radiosensitivity of melanoma cells exposed to either X-rays or carbon ion beams were evaluated in cultured cells as well as xenograft tumor models. In addition, both reactive oxygen species yield and the NADPH oxidase activity were measured in the irradiated melanoma cells. It was found that the radiation-inducible overexpression of Ras-related C3 botulinum toxin substrate 2 sensitized the melanoma cells to both X-rays and carbon ion irradiation by enhancing the NADPH oxidase activity and the subsequent reactive oxygen species production. Besides, the overexpression of Ras-related C3 botulinum toxin substrate 2 enhanced the tumor-killing effect of radiotherapy in xenograft tumors significantly. The results of this study indicate that Ras-related C3 botulinum toxin substrate 2 is promising in increasing the radiosensitivity of melanoma cells, which provides experimental evidence and theoretical basis for clinical radiosensitization of the malignant melanoma. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1942-0900 1942-0994 |
| Relation: | http://dx.doi.org/10.1155/2019/5254798; https://doaj.org/toc/1942-0900; https://doaj.org/toc/1942-0994; https://doaj.org/article/48358162d46a48fe919b5798d7a7ee96 |
| DOI: | 10.1155/2019/5254798 |
| الاتاحة: | https://doi.org/10.1155/2019/5254798 https://doaj.org/article/48358162d46a48fe919b5798d7a7ee96 |
| رقم الانضمام: | edsbas.7DAB85D |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19420900 19420994 |
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| DOI: | 10.1155/2019/5254798 |