Academic Journal
Combined Therapy With Hyperbaric Oxygen and Melatonin Effectively Reduce Brain Infarct Volume and Preserve Neurological Function After Acute Ischemic Infarct in Rat
| العنوان: | Combined Therapy With Hyperbaric Oxygen and Melatonin Effectively Reduce Brain Infarct Volume and Preserve Neurological Function After Acute Ischemic Infarct in Rat |
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| المؤلفون: | Lin, Kun-Chen, Chen, Kuan-Hung, Wallace, Christopher Glenn, Chen, Yi-Ling, Ko, Sheung-Fat, Lee, Mel S, Yip, Hon-Kan |
| المساهمون: | Chang Gung Memorial Hospital |
| المصدر: | Journal of Neuropathology & Experimental Neurology ; volume 78, issue 10, page 949-960 ; ISSN 0022-3069 1554-6578 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2019 |
| الوصف: | This study tested the hypothesis that combined hyperbaric oxygen (HBO) and melatonin (Mel) was superior to either one for protecting the brain functional and parenchymal integrity from acute ischemic stroke (IS) injury. Adult-male Sprague-Dawley rats were divided into groups 1 (sham-operated control), 2 (IS), 3 (IS + HBO), 4 (IS + Mel), and 5 (IS + HBO-Mel). By day 28 after IS, the brain infarct area (BIA) was lowest in group 1, highest in group 2, significantly higher in groups 3 and 4 than in group 5, but not different between groups 3 and 4. The neurological function at day 7, 14, and 28 exhibited an opposite pattern to BIA among the 5 groups. The protein expressions of inflammatory (IL-1β/IL-6/iNOS/TNF-α/p-NF-κB), apoptotic (cleaved-caspase3/cleaved-PARP/mitochondrial Bax), mitochondrial/DNA-damaged (cytochrome-C/γ-H2AX), oxidative stress (NOX-1/NOX-2), and autophagy (i.e. ratio of CL3B-II/CL3B-I) biomarkers displayed an identical pattern of BIA among 5 groups. Cellular expressions of inflammation (F4/80+/GFAP+) and DNA-damaged biomarker (γ-H2AX+) exhibited an identical pattern, whereas the integrities of myelin sheath/neuron (MPB+/NeuN+), endothelial cell (CD31+/vWF+), and number of small vessels exhibited an opposite pattern of BIA among the 5 groups. Combined HBO-Mel therapy offered an additional benefit in protecting the brain against IS injury. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/jnen/nlz076 |
| DOI: | 10.1093/jnen/nlz076/29603353/nlz076.pdf |
| الاتاحة: | http://dx.doi.org/10.1093/jnen/nlz076 http://academic.oup.com/jnen/advance-article-pdf/doi/10.1093/jnen/nlz076/29603353/nlz076.pdf http://academic.oup.com/jnen/article-pdf/78/10/949/30026992/nlz076.pdf |
| Rights: | https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
| رقم الانضمام: | edsbas.7D608F47 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/jnen/nlz076 |
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