Academic Journal
Serum starvation affects mitochondrial metabolism of adipose-derived stem/stromal cells
| العنوان: | Serum starvation affects mitochondrial metabolism of adipose-derived stem/stromal cells |
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| المؤلفون: | C. Giannasi, S. Niada, E. Della Morte, S. R. Casati, C. De Palma, A. Brini |
| المساهمون: | C. Giannasi, S. Niada, E. Della Morte, S.R. Casati, C. De Palma, A. Brini |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2023 |
| المجموعة: | The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
| مصطلحات موضوعية: | mesenchymal stromal cell, metabolomic, mitochondria, oxygen consumption rate, secretome, serum deprivation, serum-free, Settore BIO/14 - Farmacologia, Settore BIO/13 - Biologia Applicata |
| الوصف: | Background aims: A large part of mesenchymal stromal cell (MSC) regenerative and immunomodulatory action is mediated by paracrine signaling. Hence, an increasing body of evidence acknowledges the potential of MSC secretome in a variety of preclinical and clinical scenarios. Mid-term serum deprivation is a common approach in the pipeline of MSC secretome production. Nevertheless, up to now, little is known about the impact of this procedure on the metabolic status of donor cells. Methods: Here, through untargeted differential metabolomics, we revealed an impairment of mitochondrial metabolism in adipose-derived MSCs exposed for 72 h to serum deprivation. Results: This evidence was further confirmed by the significant accumulation of reactive oxygen species and the reduction of succinate dehydrogenase activity. Probably as a repair mechanism, an upregulation of mitochondrial superoxide dismutase was also induced. Conclusions: Of note, the analysis of mitochondrial functionality indicated that, despite a significant reduction of basal respiration and ATP production, serum-starved MSCs still responded to changes in energy demand. This metabolic phenotype correlates with the obtained evidence of mitochondrial elongation and branching upon starvation. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/37061899; info:eu-repo/semantics/altIdentifier/wos/WOS:001016756900001; firstpage:1; lastpage:8; numberofpages:8; journal:CYTOTHERAPY; https://hdl.handle.net/2434/965416; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85152456543 |
| DOI: | 10.1016/j.jcyt.2023.03.004 |
| الاتاحة: | https://hdl.handle.net/2434/965416 https://doi.org/10.1016/j.jcyt.2023.03.004 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.7D52CD8E |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.jcyt.2023.03.004 |
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