Academic Journal
Evaluating Temperature Effects on Bluetongue Virus Serotype 10 and 17 Coinfection in Culicoides sonorensis
| العنوان: | Evaluating Temperature Effects on Bluetongue Virus Serotype 10 and 17 Coinfection in Culicoides sonorensis |
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| المؤلفون: | Molly Carpenter, Jennifer Kopanke, Justin Lee, Case Rodgers, Kirsten Reed, Tyler J. Sherman, Barbara Graham, Lee W. Cohnstaedt, William C. Wilson, Mark Stenglein, Christie Mayo |
| المصدر: | International Journal of Molecular Sciences, Vol 25, Iss 5, p 3063 (2024) |
| بيانات النشر: | MDPI AG |
| سنة النشر: | 2024 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | bluetongue virus, co-infection, Culicoides, next-generation sequencing, reassortment, temperature, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Bluetongue virus (BTV) is a segmented, double-stranded RNA virus transmitted by Culicoides midges that infects ruminants. As global temperatures increase and geographical ranges of midges expand, there is increased potential for BTV outbreaks from incursions of novel serotypes into endemic regions. However, an understanding of the effect of temperature on reassortment is lacking. The objectives of this study were to compare how temperature affected Culicoides survival, virogenesis, and reassortment in Culicoides sonorensis coinfected with two BTV serotypes. Midges were fed blood meals containing BTV-10, BTV-17, or BTV serotype 10 and 17 and maintained at 20 °C, 25 °C, or 30 °C. Midge survival was assessed, and pools of midges were collected every other day to evaluate virogenesis of BTV via qRT-PCR. Additional pools of coinfected midges were collected for BTV plaque isolation. The genotypes of plaques were determined using next-generation sequencing. Warmer temperatures impacted traits related to vector competence in offsetting ways: BTV replicated faster in midges at warmer temperatures, but midges did not survive as long. Overall, plaques with BTV-17 genotype dominated, but BTV-10 was detected in some plaques, suggesting parental strain fitness may play a role in reassortment outcomes. Temperature adds an important dimension to host–pathogen interactions with implications for transmission and evolution. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/25/5/3063; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067; https://doaj.org/article/9e2a9127ce0f4645a825a4d6f6514572 |
| DOI: | 10.3390/ijms25053063 |
| الاتاحة: | https://doi.org/10.3390/ijms25053063 https://doaj.org/article/9e2a9127ce0f4645a825a4d6f6514572 |
| رقم الانضمام: | edsbas.7D2A1C68 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms25053063 |