التفاصيل البيبلوغرافية
| العنوان: |
Cell-penetration assays in the presence of inhibitors of endocytosis. |
| المؤلفون: |
Schuyler B. Gentry (11378770), Scott J. Nowak (11378773), Xuelei Ni (11378776), Stephanie A. Hill (11378779), Lydia R. Wade (11378782), William R. Clark (2903765), Aidan P. Keelaghan (11378785), Daniel P. Morris (449127), Jonathan L. McMurry (779952) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Cell Biology, Genetics, Molecular Biology, Marine Biology, Inorganic Chemistry, Biological Sciences not elsewhere classified, Physical Sciences not elsewhere classified, punctate localization consistent, heterocephalus glaber <, ab initio <, endosomal escape problem, efficiently deliver cargos, control experiments showed, div >< p, 400 nm cargo, mediated cargo delivery, penetration experiments, nm concentrations, mediated delivery, endosomal entrapment, containing cargos, cargo molecules, cargo complexes, “ tat, transporting molecules, superior internalization, remain entrapped, penetrating peptides |
| الوصف: |
WT TAT-NMR-CaM (1uM) and CBS-MBP (1uM) delivered to BHK21 cells in endpoint static assay (N = 3). Cell nuclei (blue) stained with NucBlue and CBS-MBP (red) labeled with DyLight 650. Cells pretreated with inhibitor for 30 minutes. Proteins introduced to cells in presence of inhibitor. (A) No inhibitor. (B) Chlorpromazine Hydrochloride mediated inhibition of CME. (C) Methyl-β-cyclodextrin mediated inhibition of CvME. (D) 5-(N-Ethyl-N-isopropyl) amiloride mediated inhibition of MP. Cells imaged with 40X objective and scale bar = 20 μm. Red intensity values increased to 45,000 on histogram in Zen Software to improve visualization of uptake. |
| نوع الوثيقة: |
still image |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/figure/Cell-penetration_assays_in_the_presence_of_inhibitors_of_endocytosis_/16562332 |
| DOI: |
10.1371/journal.pone.0254468.g008 |
| الاتاحة: |
https://doi.org/10.1371/journal.pone.0254468.g008 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.7D219752 |
| قاعدة البيانات: |
BASE |