Academic Journal
In vivo biotransformations of indium phosphide quantum dots revealed by X-ray microspectroscopy
| العنوان: | In vivo biotransformations of indium phosphide quantum dots revealed by X-ray microspectroscopy |
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| المؤلفون: | Veronesi, Giulia, Ruíz del Árbol, María, Castillo-Michel, Hiram, Mattera, Lucia, Onorato, Giada, Wegner, Karl David, Li Ling, Wai, Reiss, Peter, Tortiglione, Claudia |
| المساهمون: | Agence Nationale de la Recherche (France), European Commission, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Veronesi, Giulia, Reiss, Peter, Wegner, Karl David, Li Ling, Wai, Tortiglione, Claudia |
| بيانات النشر: | American Chemical Society |
| سنة النشر: | 2019 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Quantum dots, Indium phosphide, Hydra vulgaris, X-ray absorption spectroscopy |
| الوصف: | Many attempts have been made to synthesize cadmium-free quantum dots (QDs), using nontoxic materials, while preserving their unique optical properties. Despite impressive advances, gaps in knowledge of their intracellular fate, persistence, and excretion from the targeted cell or organism still exist, precluding clinical applications. In this study, we used a simple model organism (Hydra vulgaris) presenting a tissue grade of organization to determine the biodistribution of indium phosphide (InP)-based QDs by X-ray fluorescence imaging. By complementing elemental imaging with In L-edge X-ray absorption near edge structure, unique information on in situ chemical speciation was obtained. Unexpectedly, spectral profiles indicated the appearance of In-O species within the first hour post-treatment, suggesting a fast degradation of the InP QD core in vivo, induced mainly by carboxylate groups. Moreover, no significant difference in the behavior of bare core QDs and QDs capped with an inorganic Zn(Se,S) gradient shell was observed. The results paralleled those achieved by treating animals with an equivalent dose of indium salts, confirming the preferred bonding type of In3+ ions in Hydra tissues. In conclusion, by focusing on the chemical identity of indium along a 48 h long journey of QDs in Hydra, we describe a fast degradation process, in the absence of evident toxicity. These data pave the way to new paradigms to be considered in the biocompatibility assessment of QD-based biomedical applications, with greater emphasis on the dynamics of in vivo biotransformations, and suggest strategies to drive the design of future applied materials for nanotechnology-based diagnosis and therapeutics. ; K.D.W. and L.M. thank the Labex SERENADE (grant Saquado) and the French National Research Agency ANR (grant no. ANR-12-NANO-0007-03 NanoFRET) for funding. P.R. acknowledges financial support from Labex ARCANE (grant QDPhotocat), from CAPES/COFECUB (grant 858/15), and from ANR (grant number ANR-16-CE09-0015-03 Neutrinos). M.M. ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1944-8244 1944-8252 |
| Relation: | #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/EC/H2020/660228; Postprint; https://doi.org/10.1021/acsami.9b15433; Sí; ACS Applied Materials and Interfaces 11(39): 35630-35640 (2019); http://hdl.handle.net/10261/202229; http://dx.doi.org/10.13039/501100001665; http://dx.doi.org/10.13039/501100000780; http://dx.doi.org/10.13039/501100002322 |
| DOI: | 10.1021/acsami.9b15433 |
| DOI: | 10.13039/501100001665 |
| DOI: | 10.13039/501100000780 |
| DOI: | 10.13039/501100002322 |
| الاتاحة: | http://hdl.handle.net/10261/202229 https://doi.org/10.1021/acsami.9b15433 https://doi.org/10.13039/501100001665 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100002322 |
| Rights: | open |
| رقم الانضمام: | edsbas.7CD7DB16 |
| قاعدة البيانات: | BASE |
| تدمد: | 19448244 19448252 |
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| DOI: | 10.1021/acsami.9b15433 |