Academic Journal
Unravelling Novel SCN5A Mutations Linked to Brugada Syndrome: Functional, Structural, and Genetic Insights
| العنوان: | Unravelling Novel SCN5A Mutations Linked to Brugada Syndrome: Functional, Structural, and Genetic Insights |
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| المؤلفون: | Frosio A., Micaglio E., Polsinelli I., Calamaio S., Melgari D., Prevostini R., Ghiroldi A., Binda A., Carrera P., Villa M., Mastrocinque F., Presi S., Salerno R., Boccellino A., Anastasia L., Ciconte G., Ricagno S., Pappone C., Rivolta I. |
| المساهمون: | Frosio, A., Micaglio, E., Polsinelli, I., Calamaio, S., Melgari, D., Prevostini, R., Ghiroldi, A., Binda, A., Carrera, P., Villa, M., Mastrocinque, F., Presi, S., Salerno, R., Boccellino, A., Anastasia, L., Ciconte, G., Ricagno, S., Pappone, C., Rivolta, I. |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute (MDPI) |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | arrhythmias, automated patch clamp, Brugada syndrome, mexiletine, Nav1.5, SCN5A, sodium channel, sudden cardiac death |
| الوصف: | Brugada Syndrome (BrS) is a rare inherited cardiac arrhythmia causing potentially fatal ventricular tachycardia or fibrillation, mainly occurring during rest or sleep in young individuals without heart structural issues. It increases the risk of sudden cardiac death, and its characteristic feature is an abnormal ST segment elevation on the ECG. While BrS has diverse genetic origins, a subset of cases can be conducted to mutations in the SCN5A gene, which encodes for the Nav1.5 sodium channel. Our study focused on three novel SCN5A mutations (p.A344S, p.N347K, and p.D349N) found in unrelated BrS families. Using patch clamp experiments, we found that these mutations disrupted sodium currents: p.A344S reduced current density, while p.N347K and p.D349N completely abolished it, leading to altered voltage dependence and inactivation kinetics when co-expressed with normal channels. We also explored the effects of mexiletine treatment, which can modulate ion channel function. Interestingly, the p.N347K and p.D349N mutations responded well to the treatment, rescuing the current density, while p.A344S showed a limited response. Structural analysis revealed these mutations were positioned in key regions of the channel, impacting its stability and function. This research deepens our understanding of BrS by uncovering the complex relationship between genetic mutations, ion channel behavior, and potential therapeutic interventions. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/37894777; info:eu-repo/semantics/altIdentifier/wos/WOS:001167424800001; volume:24; issue:20; journal:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; https://hdl.handle.net/20.500.11768/166857 |
| DOI: | 10.3390/ijms242015089 |
| الاتاحة: | https://hdl.handle.net/20.500.11768/166857 https://doi.org/10.3390/ijms242015089 https://www.mdpi.com/1422-0067/24/20/15089 |
| Rights: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.7C818613 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/ijms242015089 |
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