Academic Journal
A pair of nonspecific phospholipases C, NPC2 and NPC6, are involved in gametophyte development and glycerolipid metabolism in Arabidopsis
| العنوان: | A pair of nonspecific phospholipases C, NPC2 and NPC6, are involved in gametophyte development and glycerolipid metabolism in Arabidopsis |
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| المؤلفون: | Ngo, Anh H., Lin, Ying‐Chen, Liu, Yu‐chi, Gutbrod, Katharina, Peisker, Helga, Dörmann, Peter, Nakamura, Yuki |
| المساهمون: | Academia Sinica |
| المصدر: | New Phytologist ; volume 219, issue 1, page 163-175 ; ISSN 0028-646X 1469-8137 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2018 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Summary Phospholipases play crucial roles in plant membrane lipid homeostasis. Nonspecific phospholipase C ( NPC s) establish a unique class of phospholipases found only in plants and certain bacteria. Here, we show that two previously uncharacterized NPC isoforms, NPC 2 and NPC 6, are required for male and female gametophyte development in Arabidopsis. Double mutant plants of npc2‐1 npc6‐2 could not be retrieved because npc2‐1 npc6‐2 ovule and pollen development is affected. Genetic complementation, reciprocal crossing and microscope observation of npc2‐1/– npc6‐2/+ and npc2‐1/+ npc6‐2/– plants suggest that NPC 2 and NPC 6 are redundant and are required for normal gametophyte development. Both NPC 2 and NPC 6 proteins are localized to the plastids. Promoter‐ GUS assays in transgenic Arabidopsis revealed that NPC 2 and NPC 6 are preferentially expressed in floral organs rather than in leaves. In vitro enzyme assays showed that NPC 2 and NPC 6 hydrolyze phosphatidylcholine and phosphatidylethanolamine, but not phosphatidate, being consistent with the reported substrate selectivity of NPC s. The amounts of phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol were increased in buds but not in flowers of npc2‐1/– npc6‐2/+ and npc2‐1/+ npc6‐2/– plants, presumably due to reduced phospholipid hydrolysis activity in developing flowers. Our results demonstrate that NPC2 and NPC6 play crucial roles in gametogenesis during flower development. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1111/nph.15147 |
| الاتاحة: | http://dx.doi.org/10.1111/nph.15147 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fnph.15147 https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.15147 |
| Rights: | http://onlinelibrary.wiley.com/termsAndConditions#vor |
| رقم الانضمام: | edsbas.7C12B3F6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/nph.15147 |
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