Academic Journal
Quantification of 782 Plasma Peptides by Multiplexed Targeted Proteomics.
| العنوان: | Quantification of 782 Plasma Peptides by Multiplexed Targeted Proteomics. |
|---|---|
| المؤلفون: | Lesur, Antoine, Bernardin, François, KONCINA, Eric, LETELLIER, Elisabeth, Kruppa, Gary, Schmit, Pierre-Olivier, DITTMAR, Gunnar |
| المصدر: | Journal of Proteome Research, 22 (6), 1630 - 1638 (2023-06-02) |
| بيانات النشر: | American Chemical Society |
| سنة النشر: | 2023 |
| المجموعة: | University of Luxembourg: ORBilu - Open Repository and Bibliography |
| مصطلحات موضوعية: | CRC, DIA, PASEF, PRM, SIL peptides, biomarker, colorectal cancer, plasma, targeted proteomics, Peptides, Proteome, Blood Proteins, Humans, Mass Spectrometry/methods, Chromatography, Liquid, Proteomics/methods, Peptides/analysis, Mass Spectrometry, Proteomics, Chemistry (all), Biochemistry, General Chemistry, Life sciences, biophysics & molecular biology, Sciences du vivant, Biochimie, biophysique & biologie moléculaire |
| الوصف: | peer reviewed ; Blood analysis is one of the foundations of clinical diagnostics. In recent years, the analysis of proteins in blood samples by mass spectrometry has taken a jump forward in terms of sensitivity and the number of identified proteins. The recent development of parallel reaction monitoring with parallel accumulation and serial fragmentation (prm-PASEF) combines ion mobility as an additional separation dimension. This increases the proteome coverage while allowing the use of shorter chromatographic gradients. To demonstrate the method's full potential, we used an isotope-labeled synthetic peptide mix of 782 peptides, derived from 579 plasma proteins, spiked into blood plasma samples with a prm-PASEF measurement allowing the quantification of 565 plasma proteins by targeted proteomics. As a less time-consuming alternative to the prm-PASEF method, we describe guided data independent acquisition (dia)-PASEF (g-dia-PASEF) and compare its application to prm-PASEF for measuring blood plasma. To demonstrate both methods' performance in clinical samples, 20 patient plasma samples from a colorectal cancer (CRC) cohort were analyzed. The analysis identified 14 differentially regulated proteins between the CRC patient and control individual plasma samples. This shows the technique's potential for the rapid and unbiased screening of blood proteins, abolishing the need for the preselection of potential biomarker proteins. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1535-3893 1535-3907 |
| Relation: | urn:issn:1535-3893; urn:issn:1535-3907; https://orbilu.uni.lu/handle/10993/60672; info:hdl:10993/60672; https://orbilu.uni.lu/bitstream/10993/60672/1/Lesur%20et%20al%2c%202023.pdf; info:pmid:37011904; wos:000967572900001 |
| DOI: | 10.1021/acs.jproteome.2c00575 |
| الاتاحة: | https://orbilu.uni.lu/handle/10993/60672 https://orbilu.uni.lu/bitstream/10993/60672/1/Lesur%20et%20al%2c%202023.pdf https://doi.org/10.1021/acs.jproteome.2c00575 |
| Rights: | open access ; http://purl.org/coar/access_right/c_abf2 ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.7BC118AC |
| قاعدة البيانات: | BASE |
| تدمد: | 15353893 15353907 |
|---|---|
| DOI: | 10.1021/acs.jproteome.2c00575 |