Academic Journal
Cerebrospinal fluid-induced retardation of amyloid β aggregation correlates with Alzheimer's disease and the APOE ε4 allele
| العنوان: | Cerebrospinal fluid-induced retardation of amyloid β aggregation correlates with Alzheimer's disease and the APOE ε4 allele |
|---|---|
| المؤلفون: | Padayachee, ER, Zetterberg, H, Portelius, E, Boren, J, Molinuevo, JL, Andreasen, N, Cukalevski, R, Linse, S, Blennow, K, Andreasson, U |
| المصدر: | Brain Research , 1651 pp. 11-16. (2016) |
| بيانات النشر: | ELSEVIER SCIENCE BV |
| سنة النشر: | 2016 |
| المجموعة: | University College London: UCL Discovery |
| مصطلحات موضوعية: | Apolipoprotein ε4, High density lipoproteins, Amyloid-β, Aggregation, Thioflavin T, Neurofibrillary tangles, Kinetics, Inhibition, Cholesterol |
| الوصف: | Misfolding and aggregation of amyloid β (Aβ) are key features of Alzheimer's disease (AD) pathogenesis, but the molecular events controlling this process are not known in detail. In vivo, Aβ aggregation and plaque formation occur in the interstitial fluid of the brain extracellular matrix. This fluid communicates freely with cerebrospinal fluid (CSF). Here, we examined the effect of human CSF on Aβ aggregation kinetics in relation to AD diagnosis and carrier status of the apolipoprotein E (APOE) ε4 allele, the main genetic risk factor for sporadic AD. The aggregation of Aβ was inhibited in the presence of CSF and, surprisingly, the effect was more pronounced in APOE ε4 carriers. However, by fractionation of CSF using size exclusion chromatography, it became evident that it was not the ApoE protein itself that conveyed the inhibition, since the retarding species eluted at lower volume, corresponding to a much higher molecular weight, than ApoE monomers. Cholesterol quantification and immunoblotting identified high-density lipoprotein particles in the retarding fractions, indicating that such particles may be responsible for the inhibition. These results add information to the yet unresolved puzzle on how the risk factor of APOE ε4 functions in AD pathogenesis. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| Relation: | https://discovery.ucl.ac.uk/id/eprint/1537447/1/1-s2.0-S0006899316306515-main.pdf; https://discovery.ucl.ac.uk/id/eprint/1537447/ |
| الاتاحة: | https://discovery.ucl.ac.uk/id/eprint/1537447/1/1-s2.0-S0006899316306515-main.pdf https://discovery.ucl.ac.uk/id/eprint/1537447/ |
| Rights: | open |
| رقم الانضمام: | edsbas.7B9FFECF |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |