التفاصيل البيبلوغرافية
| العنوان: |
Isobaric Labeling Proteomics Allows a High-Throughput Investigation of Protein Corona Orientation |
| المؤلفون: |
Nara Liessi (9740771), Luca Maragliano (523114), Valentina Castagnola (2041249), Mattia Bramini (194963), Fabio Benfenati (172964), Andrea Armirotti (67038) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biochemistry, Medicine, Inorganic Chemistry, Mental Health, Plant Biology, Chemical Sciences not elsewhere classified, High-Throughput Investigation, Proteomics Allows, tryptic peptides, Protein Corona Orientation, graphene-based nanomaterials, high-throughput assessment, silico calculations, MS, bottom-up proteomics, protein corona, corona proteins, biomolecular corona, biomolecular corona composition, high-throughput fashion, isobaric tags |
| الوصف: |
The formation of the biomolecular corona represents a crucial factor in controlling the biological interactions and trafficking of nanomaterials. In this context, the availability of key epitopes exposed on the surface of the corona, and able to engage the biological machinery, is important to define the biological fate of the material. While the full biomolecular corona composition can be investigated by conventional bottom-up proteomics, the assessment of the spatial orientation of proteins in the corona in a high-throughput fashion is still challenging. In this work, we show that labeling corona proteins with isobaric tags in their native conditions and analyzing the MS/MS spectra of tryptic peptides allow an easy and high-throughput assessment of the inner/outer orientation of the corresponding proteins in the original corona. We put our results in the context of what is currently known of the protein corona of graphene-based nanomaterials. Our conclusions are in line with previous data and were confirmed by in silico calculations. |
| نوع الوثيقة: |
text |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/online_resource/Isobaric_Labeling_Proteomics_Allows_a_High-Throughput_Investigation_of_Protein_Corona_Orientation/14512053 |
| DOI: |
10.1021/acs.analchem.0c03134.ls01 |
| الاتاحة: |
https://doi.org/10.1021/acs.analchem.0c03134.ls01 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.7B857322 |
| قاعدة البيانات: |
BASE |