Academic Journal
Epidemiology and molecular characterization of avian influenza A viruses H5N1 and H3N8 subtypes in poultry farms and live bird markets in Bangladesh
| العنوان: | Epidemiology and molecular characterization of avian influenza A viruses H5N1 and H3N8 subtypes in poultry farms and live bird markets in Bangladesh |
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| المؤلفون: | Islam, Ariful, Islam, Shariful, Flora, Meerjady, S., Amin, Emama, Woodard, Karlie, Webb, Ashley, Webster, Robert, G., Webby, Richard, J., Ducatez, Mariette, F., Hassan, Mohammad, M., El Zowalaty, Mohamed |
| المساهمون: | Deakin University Burwood, Centre for Integrative Ecology, School of Life and Environmental Sciences, EcoHealth Alliance New York, Institute of Epidemiology Disease Control and Research Dhaka, Bangladesh (IEDCR), St Jude Children's Research Hospital, Interactions hôtes-agents pathogènes Toulouse (IHAP), Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), The University of Queensland (UQ All campuses : Brisbane, Dutton Park Gatton, Herston, St Lucia and other locations ), School of veterinary science, Chittagong Veterinary and Animal Sciences University (CVASU), Higher Colleges of Technology, UAE University, This project was supported in part by funds from the University Grant Commission (UGC) of Bangladesh through Chattogram Veterinary and Animal Sciences University (CVASU), grant number UGC/CVASU#06, and by funds from the United States NIH/NIAID Centers of Excellence for Influenza Research and Surveillance (contract no. HHSN272201400008C), and by funds from the American Lebanese Syrian Associated Charities (ALSAC), St. Jude Children's Research Hospital, USA. M.E.Z. is an awardee of an NIH/NIAID/CEIRS (contract no. HHSN272201400008C) travel and research program to St. Jude CEIRS, St. Jude Children's Research Hospital, Memphis, Tennessee, USA. |
| المصدر: | ISSN: 2045-2322. |
| بيانات النشر: | HAL CCSD Nature Publishing Group |
| سنة النشر: | 2023 |
| المجموعة: | Université Toulouse III - Paul Sabatier: HAL-UPS |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio], [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
| الوصف: | International audience ; Avian influenza virus (AIV) remains a global threat, with waterfowl serving as the primary reservoir from which viruses spread to other hosts. Highly pathogenic avian influenza (HPAI) H5 viruses continue to be a devastating threat to the poultry industry and an incipient threat to humans. A cross-sectional study was conducted in seven districts of Bangladesh to estimate the prevalence and subtypes (H3, H5, and H9) of AIV in poultry and identify underlying risk factors and phylogenetic analysis of AIVs subtypes H5N1 and H3N8. Cloacal and oropharyngeal swab samples were collected from 500 birds in live bird markets (LBMs) and poultry farms. Each bird was sampled by cloacal and oropharyngeal swabbing, and swabs were pooled for further analysis. Pooled samples were analyzed for the influenza A virus (IAV) matrix (M) gene, followed by H5 and H9 molecular subtyping using real-time reverse transcription-polymerase chain reaction (rRT-PCR). Non-H5 and Non-H9 influenza A virus positive samples were sequenced to identify possible subtypes. Selected H5 positive samples were subjected to hemagglutinin (HA) and neuraminidase (NA) gene sequencing. Multivariable logistic regression was used for risk factor analysis. We found that IAV M gene prevalence was 40.20% (95% CI 35.98–44.57), with 52.38%, 46.96%, and 31.11% detected in chicken, waterfowl, and turkey, respectively. Prevalence of H5, H3, and H9 reached 22%, 3.4%, and 6.9%, respectively. Waterfowl had a higher risk of having AIV (AOR: 4.75), and H5 (AOR: 5.71) compared to chicken; more virus was detected in the winter season than in the summer season (AOR: 4.93); dead birds had a higher risk of AIVs and H5 detection than healthy birds, and the odds of H5 detection increased in LBM. All six H5N1 viruses sequenced were clade 2.3.2.1a-R1 viruses circulating since 2015 in poultry and wild birds in Bangladesh. The 12 H3N8 viruses in our study formed two genetic groups that had more similarity to influenza viruses from wild birds in Mongolia and China ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/37193732; PUBMED: 37193732; WOS: 000992335400069 |
| DOI: | 10.1038/s41598-023-33814-8 |
| الاتاحة: | https://hal.inrae.fr/hal-04163553 https://hal.inrae.fr/hal-04163553v1/document https://hal.inrae.fr/hal-04163553v1/file/2023_Islam_ScientificReports.pdf https://doi.org/10.1038/s41598-023-33814-8 |
| Rights: | http://creativecommons.org/licenses/by-nc/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.7B7B9D08 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-023-33814-8 |
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