Academic Journal
Characterisation of protein-truncating and missense variants in PALB2 in 15 768 women from Malaysia and Singapore
| العنوان: | Characterisation of protein-truncating and missense variants in PALB2 in 15 768 women from Malaysia and Singapore |
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| المؤلفون: | Ng, Pei Sze, Boonen, Rick ACM, Wijaya, Eldarina, Chong, Chan Eng, Sharma, Milan, Knaup, Sabine, Mariapun, Shivaani, Ho, Weang Kee, Lim, Joanna, Yoon, Sook-Yee, Mohd Taib, Nur Aishah, See, Mee Hoong, Li, Jingmei, Lim, Swee Ho, Tan, Ern Yu, Tan, Benita Kiat-Tee, Tan, Su-Ming, Tan, Veronique Kiat-Mien, van Dam, Rob Martinus, Rahmat, Kartini, Yip, Cheng Har, Carvalho, Sara, Luccarini, Craig, Baynes, Caroline, Dunning, Alison M, Antoniou, Antonis, van Attikum, Haico, Easton, Douglas F, Hartman, Mikael, Teo, Soo Hwang |
| المساهمون: | Wellcome Trust, Ministry of Higher Education, Malaysia, Horizon 2020 Framework Programme, National University Cancer Institute, Singapore, National Research Foundation Singapore |
| المصدر: | Journal of Medical Genetics ; volume 59, issue 5, page 481-491 ; ISSN 0022-2593 1468-6244 |
| بيانات النشر: | BMJ |
| سنة النشر: | 2021 |
| الوصف: | Background Rare protein-truncating variants (PTVs) in partner and localiser of BRCA2 ( PALB2 ) confer increased risk to breast cancer, but relatively few studies have reported the prevalence in South-East Asian populations. Here, we describe the prevalence of rare variants in PALB2 in a population-based study of 7840 breast cancer cases and 7928 healthy Chinese, Malay and Indian women from Malaysia and Singapore, and describe the functional impact of germline missense variants identified in this population. Methods Mutation testing was performed on germline DNA (n=15 768) using targeted sequencing panels. The functional impact of missense variants was tested in mouse embryonic stem cell based functional assays. Results PTVs in PALB2 were found in 0.73% of breast cancer patients and 0.14% of healthy individuals (OR=5.44; 95% CI 2.85 to 10.39, p<0.0001). In contrast, rare missense variants in PALB2 were not associated with increased risk of breast cancer. Whereas PTVs were associated with later stage of presentation and higher-grade tumours, no significant association was observed with missense variants in PALB2 . However, two novel rare missense variants (p.L1027R and p.G1043V) produced unstable proteins and resulted in a decrease in homologous recombination-mediated repair of DNA double-strand breaks. Conclusion Despite genetic and lifestyle differences between Asian and other populations, the population prevalence of PALB2 PTVs and associated relative risk of breast cancer, are similar to those reported in European populations. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1136/jmedgenet-2020-107471 |
| الاتاحة: | http://dx.doi.org/10.1136/jmedgenet-2020-107471 https://syndication.highwire.org/content/doi/10.1136/jmedgenet-2020-107471 |
| Rights: | http://creativecommons.org/licenses/by-nc/4.0/ |
| رقم الانضمام: | edsbas.7B761D70 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1136/jmedgenet-2020-107471 |
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