التفاصيل البيبلوغرافية
| العنوان: |
Metabolic profiling of T-cell growth in gas permeable membrane bioreactor ; Μεταβολικό Προφίλ της ανάπτυξης των T-λεμφοκυττάρων σε βιοαντιδραστήρα με διαπερατή στον αέρα μεμβράνη |
| المؤلفون: |
Τσίγαλου, Αικατερίνη |
| المساهمون: |
Tsigalou, Aikaterini |
| سنة النشر: |
2024 |
| المجموعة: |
University of Patras: Nemertes |
| مصطلحات موضوعية: |
T-cells, CAR-T cell therapy, Bioreactors, G-Rex platform, RPMI medium, Metabolic engineering, Liquid chromatography, Mass spectrometry, T-λεμφοκύτταρα, Θεραπεία CAR-T, Βιοαντιδραστήρες, G-Rex πλατφόρμα, RPMI θρεπτικό μέσο, Μεταβολική μηχανική, Χρωματογραφία υγρών, Φασματομετρία μάζας |
| الوصف: |
T lymphocytes are one of the primary lines of defense in the human body. They are immune cells with the crucial role of recognizing and eliminating pathogenic microorganisms. While each subset of differentiated T-cells has a unique specialized function, they cooperate to eradicate any foreign substance, including malignant cells (Hwang et al., 2020). This property has been acknowledged by scientists, leading to the development of a promising cancer treatment known as immunotherapy. Chimeric Antigen Receptor (CAR-T) therapy is a type of advanced immunotherapy, developed in the past thirty years (Sterner et al., 2021). Despite the plethora of applications and successful results, it still faces many challenges. As a personalized therapy, it comes with a high manufacturing cost and an extended production period. Furthermore, the treatment is not yet available for every type of cancer while simultaneously, toxicity and relapses are not a rare phenomenon among patients (X. Zhang et al., 2022). Understanding the underlying mechanisms that drive T-cell behaviour will be crucial for optimizing CAR-T cell production. Metabolomic analysis hold promises to provide significant insights into T-cell activation and growth conditions. In this framework this thesis is part of the European program AIDPATH, which enabled the collaboration between our laboratory and the University College London (UCL) research team in which T-cell metabolism is being studied to define the profiles that characterize their activation and proliferation process. The obtained metabolic profiles will form the basis for the subsequent synthesis of the CAR-T-cell’s phenotype. This contribution will serve as a binding block for the optimization of CAR-T-cell production. For the purpose of this thesis, T-cells from blood specimens of healthy donors were activated and proliferated in a gas-permeable membrane bioreactors (G-Rex) platform in RPMI medium. The T-cell cultivation was conducted according to the protocols developed in the University College London ... |
| نوع الوثيقة: |
other/unknown material |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
https://hdl.handle.net/10889/28414 |
| الاتاحة: |
https://hdl.handle.net/10889/28414 |
| Rights: |
Attribution-NonCommercial-NoDerivatives 4.0 International ; http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: |
edsbas.7B6178CB |
| قاعدة البيانات: |
BASE |