Academic Journal
Comparison of Diffusion MRI Acquisition Protocols for the In Vivo Characterization of the Mouse Spinal Cord: Variability Analysis and Application to an Amyotrophic Lateral Sclerosis Model
| العنوان: | Comparison of Diffusion MRI Acquisition Protocols for the In Vivo Characterization of the Mouse Spinal Cord: Variability Analysis and Application to an Amyotrophic Lateral Sclerosis Model |
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| المؤلفون: | Figini, M, Scotti, A, Marcuzzo, S, Bonanno, S, Padelli, F, Moreno-Manzano, V, Manuel Garcia-Verdugo, J, Bernasconi, P, Mantegazza, R, Bruzzone, MG, Zucca, I |
| المصدر: | PLoS ONE , 11 (8) , Article e0161646. (2016) |
| بيانات النشر: | PUBLIC LIBRARY SCIENCE |
| سنة النشر: | 2016 |
| المجموعة: | University College London: UCL Discovery |
| مصطلحات موضوعية: | Science & Technology, Multidisciplinary Sciences, Science & Technology - Other Topics, PRINCIPAL EIGENVECTOR MEASUREMENTS, GRADIENT SAMPLING SCHEMES, WHITE-MATTER PATHOLOGY, TO-NOISE RATIO, TENSOR MRI, FRACTIONAL ANISOTROPY, MEAN DIFFUSIVITY, APPARENT DIFFUSION, LONGITUDINAL MRI, AXONAL INJURY |
| الوصف: | Diffusion-weighted Magnetic Resonance Imaging (dMRI) has relevant applications in the microstructural characterization of the spinal cord, especially in neurodegenerative diseases. Animal models have a pivotal role in the study of such diseases; however, in vivo spinal dMRI of small animals entails additional challenges that require a systematical investigation of acquisition parameters. The purpose of this study is to compare three acquisition protocols and identify the scanning parameters allowing a robust estimation of the main diffusion quantities and a good sensitivity to neurodegeneration in the mouse spinal cord. For all the protocols, the signal-to-noise and contrast-to noise ratios and the mean value and variability of Diffusion Tensor metrics were evaluated in healthy controls. For the estimation of fractional anisotropy less variability was provided by protocols with more diffusion directions, for the estimation of mean, axial and radial diffusivity by protocols with fewer diffusion directions and higher diffusion weighting. Intermediate features (12 directions, b = 1200 s/mm2) provided the overall minimum inter- and intra-subject variability in most cases. In order to test the diagnostic sensitivity of the protocols, 7 G93A-SOD1 mice (model of amyotrophic lateral sclerosis) at 10 and 17 weeks of age were scanned and the derived diffusion parameters compared with those estimated in age-matched healthy animals. The protocols with an intermediate or high number of diffusion directions provided the best differentiation between the two groups at week 17, whereas only few local significant differences were highlighted at week 10. According to our results, a dMRI protocol with an intermediate number of diffusion gradient directions and a relatively high diffusion weighting is optimal for spinal cord imaging. Further work is needed to confirm these results and for a finer tuning of acquisition parameters. Nevertheless, our findings could be important for the optimization of acquisition protocols for ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| Relation: | https://discovery.ucl.ac.uk/id/eprint/10058787/1/Figini_journal.pone.0161646.PDF; https://discovery.ucl.ac.uk/id/eprint/10058787/ |
| الاتاحة: | https://discovery.ucl.ac.uk/id/eprint/10058787/1/Figini_journal.pone.0161646.PDF https://discovery.ucl.ac.uk/id/eprint/10058787/ |
| Rights: | open |
| رقم الانضمام: | edsbas.7B191A |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |