التفاصيل البيبلوغرافية
| العنوان: |
胶毒素与BSA的相互作用 ; Study on the Interaction of Gliotoxin with BSA |
| المؤلفون: |
陈俊杰, 杨梅, 张连茹, 郑忠辉, 宋思扬 |
| سنة النشر: |
2009 |
| المجموعة: |
Xiamen University Institutional Repository |
| مصطلحات موضوعية: |
胶毒素, 荧光光谱, 圆二色光谱, 紫外—可见吸收光谱, Gliotoxin, Fluorescent spectrum, CD specrum, UV-Vis spectrum |
| الوصف: |
应用荧光、圆二色和紫外—可见吸收等波谱法研究胶毒素与牛血清白蛋白(bSA)的相互作用。荧光光谱实验结果表明胶毒素主要靠疏水作用与bSA结合,而对其内源荧光产生猝灭作用,其淬灭方式为静态猝灭,胶毒素与bSA的结合常数为7.2x103l/MOl。圆二色光谱检测发现,随着胶毒素浓度的增加,bSA的α-螺旋数量也增加,当胶毒素浓度为bSA浓度的100倍时,bSA的α-螺旋增加40.1%,表明胶毒素与bSA的结合改变了bSA的空间构象。 ; The interaction between Gliotoxin and bovine serum albumin (BSA) was studied by the fluo-rescence, Circular Dichroism (CD) and ultraviolet visible (UV-Vis) techniques.The fluorescent experiment showed that the intrinsic fluorescence of BSA was quenched by the binding of gliotoxin in a static quenching procedure, with an association constant of 7.2×103 L/mol and in hydropobic forces.And the CD spectrum revealed that gliotoxin effected the conformation of BSA by increased the mass of α-helix. ; 国家863计划项目(No.2006AA09Z410) |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
Chinese |
| تدمد: |
0253-2654 |
| Relation: |
微生物学通报,2009,(8):131-135; WSWT200908032; http://dspace.xmu.edu.cn/handle/2288/118896 |
| الاتاحة: |
http://dspace.xmu.edu.cn/handle/2288/118896 |
| رقم الانضمام: |
edsbas.7A93B230 |
| قاعدة البيانات: |
BASE |