Academic Journal
Quantitative comparison of spinning disk geometries for PAINT based super-resolution microscopy.
| العنوان: | Quantitative comparison of spinning disk geometries for PAINT based super-resolution microscopy. |
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| المؤلفون: | Sirinakis, George, Allgeyer, Edward S, Cheng, Jinmei, St Johnston, Daniel |
| المصدر: | essn: 2156-7085 ; nlmid: 101540630 |
| بيانات النشر: | Optica Publishing Group //doi.org/10.1364/boe.459490 Biomed Opt Express |
| سنة النشر: | 2022 |
| المجموعة: | Apollo - University of Cambridge Repository |
| مصطلحات موضوعية: | 32 Biomedical and Clinical Sciences, 40 Engineering, 4003 Biomedical Engineering, 51 Physical Sciences, 3212 Ophthalmology and Optometry, 5102 Atomic, Molecular and Optical Physics, Bioengineering, 1.1 Normal biological development and functioning, 1.5 Resources and infrastructure (underpinning), Generic health relevance |
| الوصف: | PAINT methods that use DNA- or protein- based exchangeable probes have become popular for super-resolution imaging and have been combined with spinning disk confocal microscopy for imaging thicker samples. However, the widely available spinning disks used for routine biological imaging are not optimized for PAINT-based applications and may compromise resolution and imaging speed. Here, we use Drosophila egg chambers in the presence of the actin-binding peptide Lifeact to study the performance of four different spinning disk geometries. We find that disk geometries with higher light collection efficiency perform better for PAINT-based super-resolution imaging due to increased photon numbers and, subsequently, detection of more blinking events. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | https://www.repository.cam.ac.uk/handle/1810/341302 |
| DOI: | 10.17863/CAM.88726 |
| الاتاحة: | https://www.repository.cam.ac.uk/handle/1810/341302 https://doi.org/10.17863/CAM.88726 |
| Rights: | Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.7A4DA7B9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.17863/CAM.88726 |
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