Academic Journal
Glycosylation of the dengue 2 virus E protein at N67 is critical for virus growth in vitro but not for growth in intrathoracically inoculated Aedes aegypti mosquitoes.
| العنوان: | Glycosylation of the dengue 2 virus E protein at N67 is critical for virus growth in vitro but not for growth in intrathoracically inoculated Aedes aegypti mosquitoes. |
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| المؤلفون: | Bryant, J, Calvert, A, Mesesan, K, Crabtree, M, Volpe, K, Silengo, S, Kinney, R, Huang, C, Miller, B, Roehrig, J |
| سنة النشر: | 2016 |
| المجموعة: | Oxford University Research Archive (ORA) |
| الوصف: | To determine the importance of dengue 2 virus (DEN2V) envelope (E) protein glycosylation, virus mutants in one or both of the N-linked glycosylation motifs were prepared. We found that while the E2 mutant virus (N153Q) replicated in mammalian and mosquito cells, the E1 (N67Q) and E1/2 (N67Q and N153Q) mutant viruses were unable to grow in mammalian cells. Infection of C6/36 mosquito cells with either the E1 or E1/2 mutants resulted in the introduction of a compensatory mutation, K64N, restoring glycosylation in the area. All mutants replicated similarly in inoculated Aedes aegypti mosquitoes, with no change in their mutations. These results suggest that N-linked glycosylation of the E protein is not necessary for DEN2V replication in mosquitoes, however N-linked glycosylation at amino acid N67 (or nearby N64) is critical for the survival of the virus in either mammalian or insect cell culture. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | https://ora.ox.ac.uk/objects/uuid:eafbb9a7-8765-41c7-b513-26876dc9bc25; https://doi.org/10.1016/j.virol.2007.05.007 |
| DOI: | 10.1016/j.virol.2007.05.007 |
| الاتاحة: | https://doi.org/10.1016/j.virol.2007.05.007 https://ora.ox.ac.uk/objects/uuid:eafbb9a7-8765-41c7-b513-26876dc9bc25 |
| Rights: | info:eu-repo/semantics/embargoedAccess |
| رقم الانضمام: | edsbas.7A1DFA31 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.virol.2007.05.007 |
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