Academic Journal
Assessment of trimethoprim-sulfamethoxazole susceptibility testing methods for fastidious Haemophilus spp
| العنوان: | Assessment of trimethoprim-sulfamethoxazole susceptibility testing methods for fastidious Haemophilus spp |
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| المؤلفون: | Sierra, Y., Tubau, F., González-Díaz, A., Carrera-Salinas, A., Moleres, J., Bajanca-Lavado, P., Garmendia, J., Domínguez, M. Ángeles, Ardanuy, C., Martí, S. |
| بيانات النشر: | Elsevier/ European Society of Clinical Microbiology and Infectious Diseases |
| سنة النشر: | 2020 |
| المجموعة: | National Health Institute, Portugal: Repositório Científico |
| مصطلحات موضوعية: | Antimicrobial Susceptibility Testing Methods, Trimethoprim-sulfamethoxazole, Haemophilus Parainfluenzae, Eucast Breakpoints, Resistance-related Determinants, Haemophilus Influenzae, Clinical Resistance Breakpoint, Infecções Respiratórias |
| الوصف: | To compare the determinants of trimethoprim-sulfamethoxazole resistance with established susceptibility values for fastidious Haemophilus spp., to provide recommendations for optimal trimethoprim-sulfamethoxazole measurement. We collected 50 strains each of Haemophilus influenzae and Haemophilus parainfluenzae at Bellvitge University Hospital. Trimethoprim-sulfamethoxazole susceptibility was tested by microdilution, E-test and disc diffusion using both Mueller-Hinton fastidious (MH-F) medium and Haemophilus test medium (HTM) following EUCAST and CLSI criteria, respectively. Mutations in folA, folP and additional determinants of resistance were identified in whole-genome-sequenced isolates. Strains presented generally higher rates of trimethoprim-sulfamethoxazole resistance when grown on HTM than on MH-F, independent of the methodology used (average MIC 2.6-fold higher in H. influenzae and 1.2-fold higher in H. parainfluenzae). The main resistance-related determinants were as follows: I95L and F154S/V in folA; 3- and 15-bp insertions and substitutions in folP; acquisition of sul genes; and FolA overproduction potentially linked to mutations in -35 and -10 promoter motifs. Of note, 2 of 19 H. influenzae strains (10.5%) and 9 of 33 H. parainfluenzae strains (27.3%) with mutations and assigned as resistant by microdilution were inaccurately considered susceptible by disc diffusion. This misinterpretation was resolved by raising the clinical resistance breakpoint of the EUCAST guidelines to ≤30 mm. Given the routine use of disc diffusion, a significant number of strains could potentially be miscategorized as susceptible to trimethoprim-sulfamethoxazole despite having resistance-related mutations. A simple modification to the current clinical resistance breakpoint given by the EUCAST guideline for MH-F ensures correct interpretation and correlation with the reference standard method of microdilution. ; This study has been funded by Instituto de Salud Carlos III through the Projects from the Fondo de Investigaciones ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1198-743X |
| Relation: | https://linkinghub.elsevier.com/retrieve/pii/S1198-743X(19)30624-X; Clin Microbiol Infect. 2019 Dec 4;S1198-743X(19)30624-X. doi:10.1016/j.cmi.2019.11.022. Online ahead of print.; http://hdl.handle.net/10400.18/6475 |
| DOI: | 10.1016/j.cmi.2019.11.022 |
| الاتاحة: | http://hdl.handle.net/10400.18/6475 https://doi.org/10.1016/j.cmi.2019.11.022 |
| Rights: | openAccess |
| رقم الانضمام: | edsbas.7A126AF6 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 1198743X |
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| DOI: | 10.1016/j.cmi.2019.11.022 |