التفاصيل البيبلوغرافية
| العنوان: |
CHARMM-GUI Membrane Builder for Lipid Nanoparticles with Ionizable Cationic Lipids and PEGylated Lipids |
| المؤلفون: |
Soohyung Park (1325910), Yeol Kyo Choi (3913363), Seonghoon Kim (1559083), Jumin Lee (1372398), Wonpil Im (79057) |
| سنة النشر: |
1753 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Cell Biology, Genetics, Pharmacology, Biotechnology, Computational Biology, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, two previous studies, preferentially extended laterally, art delivery system, small interfering rna, new lipid types, membrane builder <, existing lipid types, lipids also relaxes, realistic lnp patch, rational lnp design, cationic lipids fit, cationic lipids due, gui membrane builder, gains tremendous interest, ), containing lnp membranes, ionizable cationic lipids, without genetic drugs, cationic lipids, genetic drugs, |
| الوصف: |
A lipid nanoparticle (LNP) formulation is a state-of-the-art delivery system for genetic drugs such as DNA, messenger RNA, and small interfering RNA, which is successfully applied to COVID-19 vaccines and gains tremendous interest in therapeutic applications. Despite its importance, a molecular-level understanding of the LNP structures and dynamics is still lacking, which makes rational LNP design almost impossible. In this work, we present an extension of CHARMM-GUI Membrane Builder to model and simulate all-atom LNPs with various (ionizable) cationic lipids and PEGylated lipids (PEG-lipids). These new lipid types can be mixed with any existing lipid types with or without a biomolecule of interest, and the generated systems can be simulated using various molecular dynamics engines. As a first illustration, we considered model LNP membranes with DLin-KC2-DMA (KC2) or DLin-MC3-DMA (MC3) without PEG-lipids. The results from these model membranes are consistent with those from the two previous studies, albeit with mild accumulation of neutral MC3 in the bilayer center. To demonstrate Membrane Builder ’s capability of building a realistic LNP patch, we generated KC2- or MC3-containing LNP membranes with high concentrations of cholesterol and ionizable cationic lipids together with 2 mol % PEG-lipids. We observe that PEG-chains are flexible, which can be more preferentially extended laterally in the presence of cationic lipids due to the attractive interactions between their head groups and PEG oxygen. The presence of PEG-lipids also relaxes the lateral packing in LNP membranes, and the area compressibility modulus ( K A ) of LNP membranes with cationic lipids fit into typical K A of fluid-phase membranes. Interestingly, the interactions between PEG oxygen and the head group of ionizable cationic lipids induce a negative curvature. We hope that this LNP capability in Membrane Builder can be useful to better characterize various LNPs with or without genetic drugs for rational LNP design. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/CHARMM-GUI_Membrane_Builder_for_Lipid_Nanoparticles_with_Ionizable_Cationic_Lipids_and_PEGylated_Lipids/16652955 |
| DOI: |
10.1021/acs.jcim.1c00770.s001 |
| الاتاحة: |
https://doi.org/10.1021/acs.jcim.1c00770.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.79BC28DF |
| قاعدة البيانات: |
BASE |