التفاصيل البيبلوغرافية
| العنوان: |
Large-scale voltage imaging in behaving mice using targeted illumination |
| المؤلفون: |
Xiao, Sheng, Lowet, Eric, Gritton, Howard J, Fabris, Pierre, Wang, Yangyang, Sherman, Jack, Mount, Rebecca A, Tseng, Hua-an, Man, Heng-Ye, Straub, Christoph, Piatkevich, Kiryl D, Boyden, Edward S, Mertz, Jerome, Han, Xue |
| المساهمون: |
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences |
| المصدر: |
Elsevier |
| بيانات النشر: |
Elsevier BV |
| سنة النشر: |
2023 |
| المجموعة: |
DSpace@MIT (Massachusetts Institute of Technology) |
| الوصف: |
Recent improvements in genetically encoded voltage indicators enabled optical imaging of action potentials and subthreshold transmembrane voltage in vivo. To perform high-speed voltage imaging of many neurons simultaneously over a large anatomical area, widefield microscopy remains an essential tool. However, the lack of optical sectioning makes widefield microscopy prone to background cross-contamination. We implemented a digital-micromirror-device-based targeted illumination strategy to restrict illumination to the cells of interest and quantified the resulting improvement both theoretically and experimentally with SomArchon expressing neurons. We found that targeted illumination increased SomArchon signal contrast, decreased photobleaching, and reduced background cross-contamination. With the use of a high-speed, large-area sCMOS camera, we routinely imaged tens of spiking neurons simultaneously over minutes in behaving mice. Thus, the targeted illumination strategy described here offers a simple solution for widefield voltage imaging of many neurons over a large field of view in behaving animals. |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
iScience; https://hdl.handle.net/1721.1/148692; Xiao, Sheng, Lowet, Eric, Gritton, Howard J, Fabris, Pierre, Wang, Yangyang et al. 2021. "Large-scale voltage imaging in behaving mice using targeted illumination." iScience, 24 (11). |
| الاتاحة: |
https://hdl.handle.net/1721.1/148692 |
| Rights: |
Creative Commons Attribution-NonCommercial-NoDerivs License ; http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| رقم الانضمام: |
edsbas.799FB577 |
| قاعدة البيانات: |
BASE |