Academic Journal
In vitro selection of RNA aptamers against a composite small molecule-protein surface
| العنوان: | In vitro selection of RNA aptamers against a composite small molecule-protein surface |
|---|---|
| المؤلفون: | Kelly A. Plummer, James M. Carothers, Masahiro Yoshimura, Jack W. Szostak, Gregory L. Verdine |
| المساهمون: | The Pennsylvania State University CiteSeerX Archives |
| المصدر: | http://nar.oxfordjournals.org/content/33/17/5602.full.pdf. |
| سنة النشر: | 2005 |
| المجموعة: | CiteSeerX |
| الوصف: | A particularly challenging problem in chemical biology entails developing systems for modulating the activity of RNA using small molecules. One prom-ising new approach towards this problem exploits the phenomenon of ‘surface borrowing, ’ in which the small molecule is presented to the RNA in complex with a protein, thereby expanding the overall surface area available for interaction with RNA. To extend the utility of surface borrowing to include potential applications in synthetic biology, we set out to create an ‘orthogonal ’ RNA-targeting system, one in which all components are foreign to the cell. Here we report the identification of small RNA modules selected in vitro to bind a surface-engineered protein, but only when the two macromolecules are bound to a syn-thetic bifunctional small molecule. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | English |
| Relation: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.516.1060; http://nar.oxfordjournals.org/content/33/17/5602.full.pdf |
| الاتاحة: | http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.516.1060 http://nar.oxfordjournals.org/content/33/17/5602.full.pdf |
| Rights: | Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| رقم الانضمام: | edsbas.796CCEC8 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |