التفاصيل البيبلوغرافية
| العنوان: |
Stimulation of Glutathione Depletion, Ros Production and Cell Cycle Arrest of Dental Pulp Cells and Gingival Epithelial Cells by Hema |
| المؤلفون: |
張曉華, 郭敏光, 張美姬, 黃桂芬, 王姻麟, 王若松, 鄭景暉, CHANG, HSIAO-HUA, GUO, MING-KUANG, FREDERICK H. KASTEN, CHANG, MEI-CHI, HUANG, GUAY-FEN, WANG, YIN-LIN, WANG, JUO-SONG, JENG, JIIANG-HUEI |
| المساهمون: |
牙醫學系所 |
| سنة النشر: |
2005 |
| المجموعة: |
National Taiwan University Institutional Repository (NTUR) |
| مصطلحات موضوعية: |
apoptosis, cell cycle, glutathione, HEMA, pulp cells, gingival cells |
| الوصف: |
2-Hydroxy-ethyl methacrylate (HEMA) is the major component released from resin-modified glass ionomer cements and dental adhesives. Human tissues mainly affected by HEMA are oral epithelium and dental pulp. We treated human gingival epithelial S-G cells and pulp fibroblasts (HPF) with various concentrations of HEMA, to evaluate its effects on cell growth, cell cycle progression, intracellular glutathione ( GSH) level and reactive oxygen species (ROS) production. HEMA-induced growth inhibition in HPF and S-G cells in a dose-dependent manner, which may be partially explained by induction of cell cycle perturbation. G(2)/M phase arrest was noted after exposure of HPF to 5 and 10 mM of HEMA, concomitant with glutathione depletion and ROS production. S -phase arrest occurred in S-G cells when treated with 2.5 and 5 mm, while at 10 mm a sub-G(0)/G(1) peak was noted, indicating the potential induction of apoptosis. GSH depletion was marked in S-G cells only at concentrations of 5 and 10 mM, but excessive ROS production was noted at concentration of 1 mM and rose with dose increase between 1 and 5 mm, then lessened at 10 mm. This suggested that the increase of ROS in S-G cells was not mainly caused by GSH depletion. These results helped to define the mechanism of the cytotoxicity caused by HEMA. (C) 2004 Elsevier Ltd. All rights reserved. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| Relation: |
BIOMATERIALS v.26 n.7 pp.745-753; http://ntur.lib.ntu.edu.tw/handle/246246/92713 |
| الاتاحة: |
http://ntur.lib.ntu.edu.tw/handle/246246/92713 |
| رقم الانضمام: |
edsbas.7915653E |
| قاعدة البيانات: |
BASE |