Academic Journal
Sensitive detection of protein ubiquitylation using a protein-fragment complementation assay
| العنوان: | Sensitive detection of protein ubiquitylation using a protein-fragment complementation assay |
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| المؤلفون: | Le Boulch, Marie, Brossard, Audrey, Le Dez, Gaëlle, Léon, Sébastien, Rabut, Gwenaël |
| المساهمون: | Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut Jacques Monod (IJM (UMR_7592)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), PICS07394, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Université de Rennes 1, ANR-16-CE13-0002-02, Agence Nationale de la Recherche, Ligue Contre le Cancer, Biosit, ANR-16-CE13-0002,P-Nut,Remodelage de la membrane plasmique par la disponibilité en nutriments(2016) |
| المصدر: | ISSN: 0021-9533. |
| بيانات النشر: | HAL CCSD Company of Biologists |
| سنة النشر: | 2020 |
| المجموعة: | Université de Rennes 1: Publications scientifiques (HAL) |
| مصطلحات موضوعية: | Ubiquitin, NanoBiT, NanoLuc, PCA, Ubiquitination, UbiCREST, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] |
| الوصف: | International audience ; Ubiquitylation is a reversible post-translational protein modification that regulates a multitude of cellular processes. Detection of ubiquitylated proteins is often challenging, because of their low abundance. Here, we present NUbiCA, a sensitive protein-fragment complementation assay to facilitate the monitoring of ubiquitylation events in cultured cells and model organisms. Using yeast as a model system, we demonstrate that NUbiCA enables to accurately monitor mono- and poly-ubiquitylation of proteins expressed at endogenous levels. We also show that it can be applied to decipher the topology of ubiquitin conjugates. Moreover, we assembled a genome wide collection of yeast strains ready to investigate the ubiquitylation of proteins with this new assay. This resource will facilitate the analysis of local or transient ubiquitylation events that are difficult to detect with current methods. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/32409563; hal-02635146; https://univ-rennes.hal.science/hal-02635146; https://univ-rennes.hal.science/hal-02635146/document; https://univ-rennes.hal.science/hal-02635146/file/Le%20Boulch%20et%20al-2020-Sensitive%20detection%20of%20protein%20ubiquitylation.pdf; PUBMED: 32409563 |
| DOI: | 10.1242/jcs.240093 |
| الاتاحة: | https://univ-rennes.hal.science/hal-02635146 https://univ-rennes.hal.science/hal-02635146/document https://univ-rennes.hal.science/hal-02635146/file/Le%20Boulch%20et%20al-2020-Sensitive%20detection%20of%20protein%20ubiquitylation.pdf https://doi.org/10.1242/jcs.240093 |
| Rights: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.789BDF69 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1242/jcs.240093 |
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