التفاصيل البيبلوغرافية
| العنوان: |
Data_Sheet_1_Infection Kinetics and Transmissibility of a Reanimated Dengue Virus Serotype 4 Identified Originally in Wild Aedes aegypti From Florida.DOCX |
| المؤلفون: |
Jasmine B. Ayers (9449534), Xuping Xie (2922237), Heather Coatsworth (11477980), Caroline J. Stephenson (5334458), Christy M. Waits (4775040), Pei-Yong Shi (241650), Rhoel R. Dinglasan (310391) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Microbiology, Microbial Genetics, Microbial Ecology, Mycology, Aedes aegypti, mosquito, infectious clone, arbovirus, dengue virus serotype 4, transovarial transmission, vertical transmission, Florida |
| الوصف: |
Dengue virus is the most prevalent mosquito-borne virus, causing approximately 390 million infections and 25,000 deaths per year. Aedes aegypti, the primary mosquito vector of dengue virus, is well-established throughout the state of Florida, United States. Autochthonous transmission of dengue virus to humans in Florida has been increasing since 2009, alongside consistent importation of dengue cases. However, most cases of first infection with dengue are asymptomatic and the virus can be maintained in mosquito populations, complicating surveillance and leading to an underestimation of disease risk. Metagenomic sequencing of A. aegypti mosquitoes in Manatee County, Florida revealed the presence of dengue virus serotype 4 (DENV-4) genomes in mosquitoes from multiple trapping sites over 2years, in the absence of a human DENV-4 index case, and even though a locally acquired case of DENV-4 has never been reported in Florida. This finding suggested that: (i) DENV-4 may circulate among humans undetected; (ii) the virus was being maintained in the mosquito population, or (iii) the detected complete genome sequence may not represent a viable virus. This study demonstrates that an infectious clone generated from the Manatee County DENV-4 (DENV-4M) sequence is capable of infecting mammalian and insect tissue culture systems, as well as adult female A. aegypti mosquitoes when fed in a blood meal. However, the virus is subject to a dose dependent infection barrier in mosquitoes, and has a kinetic delay compared to a phylogenetically related wild-type (WT) control virus from a symptomatic child, DENV-4H (strain Homo sapiens/Haiti-0075/2015, GenBank accession MK514144.1). DENV-4M disseminates from the midgut to the ovary and saliva at 14days post-infection. Viral RNA was also detectable in the adult female offspring of DENV-4M infected mosquitoes. These results demonstrate that the virus is capable of infecting vector mosquitoes, is transmissible by bite, and is vertically transmitted, indicating a mechanism for maintenance in ... |
| نوع الوثيقة: |
dataset |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/dataset/Data_Sheet_1_Infection_Kinetics_and_Transmissibility_of_a_Reanimated_Dengue_Virus_Serotype_4_Identified_Originally_in_Wild_Aedes_aegypti_From_Florida_DOCX/16677574 |
| DOI: |
10.3389/fmicb.2021.734903.s001 |
| الاتاحة: |
https://doi.org/10.3389/fmicb.2021.734903.s001 |
| Rights: |
CC BY 4.0 |
| رقم الانضمام: |
edsbas.788920FD |
| قاعدة البيانات: |
BASE |