Academic Journal
Elementary Reactions in the Functional Triads of the Blue-Light Photoreceptor BLUF Domain
| العنوان: | Elementary Reactions in the Functional Triads of the Blue-Light Photoreceptor BLUF Domain |
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| المؤلفون: | Xiu-Wen Kang, Kailin Wang, Xiaofan Zhang, Dongping Zhong, Bei Ding |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Biophysics, Biochemistry, Medicine, Molecular Biology, Neuroscience, Evolutionary Biology, Mental Health, Infectious Diseases, Plant Biology, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, using ultrafast spectroscopy, unique bidirectional electron, nonphotosensitive protein machines, kinetic isotope effects, coupled electron transfer, photoactivation reaction cycle, three elementary processes, blue light using, photoactivation mechanism, elementary steps, elementary reactions, temporal sequence, smallest photoreceptors, recent efforts, proton relay, perspective summarizes, kies ), including proton |
| الوصف: | The blue light using the flavin (BLUF) domain is one of the smallest photoreceptors in nature, which consists of a unique bidirectional electron-coupled proton relay process in its photoactivation reaction cycle. This perspective summarizes our recent efforts in dissecting the photocycle into three elementary processes, including proton-coupled electron transfer (PCET), proton rocking, and proton relay. Using ultrafast spectroscopy, we have determined the temporal sequence, rates, kinetic isotope effects (KIEs), and concertedness of these elementary steps. Our findings provide important implications for illuminating the photoactivation mechanism of the BLUF domain and suggest an engineering platform to characterize intricate reactions involving proton motions that are ubiquitous in nonphotosensitive protein machines. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| Relation: | https://figshare.com/articles/journal_contribution/Elementary_Reactions_in_the_Functional_Triads_of_the_Blue-Light_Photoreceptor_BLUF_Domain/25273493 |
| DOI: | 10.1021/acs.jpcb.3c07988.s001 |
| الاتاحة: | https://doi.org/10.1021/acs.jpcb.3c07988.s001 https://figshare.com/articles/journal_contribution/Elementary_Reactions_in_the_Functional_Triads_of_the_Blue-Light_Photoreceptor_BLUF_Domain/25273493 |
| Rights: | CC BY-NC 4.0 |
| رقم الانضمام: | edsbas.78621E9A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.jpcb.3c07988.s001 |
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