Academic Journal
Potential mechanisms of attenuation for rifampicin-passaged strains of Flavobacterium psychrophilum ; BMC Microbiology
| العنوان: | Potential mechanisms of attenuation for rifampicin-passaged strains of Flavobacterium psychrophilum ; BMC Microbiology |
|---|---|
| المؤلفون: | Gliniewicz, Karol, Wildung, Mark, Orfe, Lisa H., Wiens, Gregory D., Cain, Kenneth D., Lahmers, Kevin K., Snekvik, Kevin R., Call, Douglas R. |
| بيانات النشر: | Biomed Central |
| سنة النشر: | 2015 |
| المجموعة: | VTechWorks (VirginiaTech) |
| مصطلحات موضوعية: | Microbiology, Rifampicin, Attenuation, Flavobacterium psychrophilum, SNP, Methylome, EDWARDSIELLA-ICTALURI VACCINE, BACTERIAL RNA-POLYMERASE, ESCHERICHIA-COLI, STAPHYLOCOCCUS-AUREUS, MYCOBACTERIUM-TUBERCULOSIS, CAROTENOID BIOSYNTHESIS, TRANSPOSON MUTAGENESIS, AEROMONAS-HYDROPHILA, ENTERIC SEPTICEMIA, CHANNEL CATFISH |
| الوصف: | Background Flavobacterium psychrophilum is the etiologic agent of bacterial coldwater disease in salmonids. Earlier research showed that a rifampicin-passaged strain of F. psychrophilum (CSF 259-93B.17) caused no disease in rainbow trout (Oncorhynchus mykiss, Walbaum) while inducing a protective immune response against challenge with the virulent CSF 25993 strain. We hypothesized that rifampicin passage leads to an accumulation of genomic mutations that, by chance, reduce virulence. To assess the pattern of phenotypic and genotypic changes associated with passage, we examined proteomic, LPS and single-nucleotide polymorphism (SNP) differences for two F. psychrophilum strains (CSF 25993 and THC 0290) that were passaged with and without rifampicin selection. Results Rifampicin resistance was conveyed by expected mutations in rpoB, although affecting different DNA bases depending on the strain. One rifampicin-passaged CSF 25993 strain (CR) was attenuated (4 % mortality) in challenged fish, but only accumulated eight nonsynonymous SNPs compared to the parent strain. A CSF 25993 strain passaged without rifampicin (CN) accumulated five nonsynonymous SNPs and was partially attenuated (28 % mortality) compared to the parent strain (54.5 % mortality). In contrast, there were no significant change in fish mortalities among THC 0290 wild-type and passaged strains, despite numerous SNPs accumulated during passage with (n=174) and without rifampicin (n=126). While only three missense SNPs were associated with attenuation, a Ser492Phe rpoB mutation in the CR strain may contribute to further attenuation. All strains except CR retained a gliding motility phenotype. Few proteomic differences were observed by 2D SDS-PAGE and there were no apparent changes in LPS between strains. Comparative methylome analysis of two strains (CR and TR) identified no shared methylation motifs for these two strains. Conclusion Multiple genomic changes arose during passage experiments with rifampicin selection pressure. Consistent with our ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 15 pages; application/pdf |
| اللغة: | English |
| Relation: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000361448300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=930d57c9ac61a043676db62af60056c1; BMC Microbiology. 2015 Sep 16;15(1):179; http://hdl.handle.net/10919/73467; 15 |
| DOI: | 10.1186/s12866-015-0518-1 |
| الاتاحة: | http://hdl.handle.net/10919/73467 https://doi.org/10.1186/s12866-015-0518-1 |
| Rights: | Creative Commons Attribution 4.0 International ; http://creativecommons.org/licenses/by/4.0/ ; Gliniewicz et al. |
| رقم الانضمام: | edsbas.77DFEBF9 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12866-015-0518-1 |
|---|