| الوصف: |
Globally, approximately 170 million people (representing approximately 3% of the population worldwide), are infected with hepatitis C virus (HCV) and at risk of serious liver disease, including chronic hepatitis. We propose a new quantum dots (QDs)-supported RNA oligonucleotide approach for the specific and sensitive detection of viral protein using a biochip. This method was developed by immobilizing a HCV nonstructural protein 5B (NS5B) on the surface of a glass chip via the formation of a covalent bond between an amine protein group and a ProLinker (TM) glass chip. The QDs-supported RNA oligonucleotide was conjugated via an amide formation reaction from coupling of a 5`-end-amine-modified RNA oligonucleotide on the surface of QDs displaying carboxyl groups via standard EDC coupling. The QDs-conjugated RNA oligonucleotide was interacted to immobilized viral protein NS5B on the biochip. The detection is based on the variation of signal of QDs-supported RNA oligonucleotide bound on an immobilized biochip. It was demonstrated that the value of the signal has a linear relationship with concentrations of the HCV NS5B viral protein in the 1 mu g mL(-1) to 1 ng mL(-1) range with a detection limit of 1 ng mL(-1). The major advantages of this RNA-oligonucleotide nanoparticle assay are its good specificity, ease of performance, and ability to perform one-spot monitoring. The proposed method could be used as a general method of HCV detection and is expected to be applicable to other types of diseases as well. ; This work was supported by SNU-KAERI Degree and Research Center for Radiation Convergence Sciences from the Korea Research Council of Foundation (KRCF) Science and Technology, South Korea. ; Griffin J, 2009, CHEM-EUR J, V15, P342, DOI 10.1002/chem.200801812 ; GHASEMI Y, 2009, ACTA BIOMED, V80, P156 ; Biju V, 2008, ANAL BIOANAL CHEM, V391, P2469, DOI 10.1007/s00216-008-2185-7 ; Medintz IL, 2008, INT J NANOMED, V3, P151 ; Riccardi CDS, 2008, ANAL CHEM, V80, P237, DOI 10.1021/ac701613t ; NEERJA KB, 2008, NUCLEIC ... |
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