Academic Journal
Light Emitting Diode Photobiomodulation Enhances Oxidative Redox Capacity in Murine Macrophages Stimulated with Bothrops jararacussu Venom and Isolated PLA 2 s
| العنوان: | Light Emitting Diode Photobiomodulation Enhances Oxidative Redox Capacity in Murine Macrophages Stimulated with Bothrops jararacussu Venom and Isolated PLA 2 s |
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| المؤلفون: | dos Reis, Valdison Pereira, da Silva Setúbal, Sulamita, Ferreira e Ferreira, Alex A., Santana, Hallison Mota, Silva, Milena Daniela Souza, Sousa, Ortência De Oliveira, Boeno, Charles Nunes, Soares, Andreimar M., Zamuner, Stella R., Zuliani, Juliana P. |
| المساهمون: | Pasciu, Valeria, Coordenaçâo de Aperfeiçoamento de Pessoal de Nível Superior |
| المصدر: | BioMed Research International ; volume 2022, issue 1 ; ISSN 2314-6133 2314-6141 |
| بيانات النشر: | Wiley |
| سنة النشر: | 2022 |
| المجموعة: | Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: | Photobiomodulation therapy associated with conventional antivenom treatment has been shown to be effective in reducing the local effects caused by bothropic venoms in preclinical studies. In this study, we analyzed the influence of photobiomodulation using light emitting diode (LED) on the oxidative stress produced by murine macrophages stimulated with Bothrops jararacussu venom and it isolated toxins BthTX‐I and BthTX‐II. Under LED treatment, we evaluated the activity of the antioxidant enzymes catalase, superoxide dismutase, and peroxidase as well as the release of hydrogen peroxide and the enzyme lactate dehydrogenase. To investigate whether NADPH oxidase complex activation and mitochondrial pathways could contribute to hydrogen peroxide production by macrophages, we tested the effect of two selective inhibitors, apocynin and CCCP3, respectively. Our results showed that LED therapy was able to decrease the production of hydrogen peroxide and the liberation of lactate dehydrogenase, indicating less cell damage. In addition, the antioxidant enzymes catalase, superoxide dismutase, and peroxidase increased in response to LED treatment. The effect of LED treatment on macrophages was inhibited by CCCP3, but not by apocynin. These findings show that LED photobiomodulation treatment protects macrophages, at least in part, by reducing oxidative stress caused B. jararacussu venom and toxins. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1155/2022/5266211 |
| الاتاحة: | https://doi.org/10.1155/2022/5266211 http://downloads.hindawi.com/journals/bmri/2022/5266211.pdf http://downloads.hindawi.com/journals/bmri/2022/5266211.xml https://onlinelibrary.wiley.com/doi/pdf/10.1155/2022/5266211 |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: | edsbas.77C74A05 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1155/2022/5266211 |
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