Academic Journal
First meiotic anaphase requires Cep55-dependent inhibitory cyclin-dependent kinase 1 phosphorylation
| العنوان: | First meiotic anaphase requires Cep55-dependent inhibitory cyclin-dependent kinase 1 phosphorylation |
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| المؤلفون: | Zhou, Chenxi, Hancock, Janelle L., Khanna, Kum Kum, Homer, Hayden A. |
| بيانات النشر: | The Company of Biologists Ltd |
| سنة النشر: | 2019 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | RESEARCH ARTICLE |
| الوصف: | During mitosis, anaphase is triggered by anaphase-promoting complex (APC)-mediated destruction of securin and cyclin B1, which leads to inactivation of cyclin-dependent kinase 1 (Cdk1). By regulating APC activity, the mitotic spindle assembly checkpoint (SAC) therefore has robust control over anaphase timing to prevent chromosome mis-segregation. Mammalian oocytes are prone to aneuploidy, the reasons for which remain obscure. In mitosis, Cep55 is required post-anaphase for the final steps of cytokinesis. We found that Cep55-depleted mouse oocytes progress normally through early meiosis I, but that anaphase I fails as a result of persistent Cdk1 activity. Unexpectedly, Cdk1 inactivation was compromised following Cep55 depletion, despite on-time SAC silencing and intact APC-mediated proteolysis. We found that impaired Cdk1 inactivation was caused by inadequate inhibitory Cdk1 phosphorylation consequent upon failure to suppress Cdc25 phosphatase, identifying a proteolysis-independent step necessary for anaphase I. Thus, the SAC in oocytes does not exert exclusive control over anaphase I initiation, providing new insight into vulnerability to error. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| Relation: | http://jcs.biologists.org/cgi/content/short/132/18/jcs233379; http://dx.doi.org/10.1242/jcs.233379 |
| DOI: | 10.1242/jcs.233379 |
| الاتاحة: | http://jcs.biologists.org/cgi/content/short/132/18/jcs233379 https://doi.org/10.1242/jcs.233379 |
| Rights: | Copyright (C) 2019, Company of Biologists |
| رقم الانضمام: | edsbas.778053D3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1242/jcs.233379 |
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