Report
Clinical evaluation of a RT-LAMP SARS-CoV-2 test for the Point-Of-Care, rapid, low-cost, integrating sample solid phase extraction and on which reagents are lyophilized
| العنوان: | Clinical evaluation of a RT-LAMP SARS-CoV-2 test for the Point-Of-Care, rapid, low-cost, integrating sample solid phase extraction and on which reagents are lyophilized |
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| المؤلفون: | Coz, E., Garneret, P., Martin, E., Do Nascimento, D., Vilquin, A., Hoinard, D., Feher, M., Grassin, Q., Vanhomwegen, J., Manuguerra, J.C, Mukherjee, S., Olivo-Marin, J.-C., Brient-Litzler, E., Merzoug, M., Collin, E., Tabeling, P., Rossi, B. |
| المساهمون: | Laboratoire Microfluidique, MEMS, Nanostructures (MMN), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Chimie-Biologie-Innovation (UMR 8231) (CBI), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Gulliver (UMR 7083), Cellule d'Intervention Biologique d'Urgence (Centre National de Référence) - Laboratory for Urgent Response to Biological Threats (National Reference Center) (CIBU), Université Paris Cité (UPCité)-Environnement et Risques infectieux - Environment and Infectious Risks (ERI), Institut Pasteur Paris (IP)-Université Paris Cité (UPCité)-Institut Pasteur Paris (IP), Analyse d'images biologiques - Biological Image Analysis (BIA), Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), Service développement de l'innovation - Innovation Office, Direction des Applications de la Recherche et des Relations industrielles - The Technology Transfer and Industrial Partnership Department (DARRI), Institut Pasteur Paris (IP)-Institut Pasteur Paris (IP), CMC Ambroise Paré Neuilly-sur-Seine, Hôpital Robert Ballanger Aulnay-sous-Bois, The authors thank La Ville de Paris, ESPCI, CNRS, PSL, its Department of Tech Transfer “PSLValorisation”, IPGG platform (ANR-10-EQPX-34), the Institut Pasteur, the institut Carnot Pasteur Maladies Infectieuses (ANR-11-CARN-017-01), the Region Ile-de-France DIM ELICIT program, the Labex IBEID (ANR-10-LABX-62-IBEID), the France BioImaging infrastructure (ANR-10-INBS-04), ANR Flash, Fonds de l’ESPCI, Bettencourt Foundation, JL Missika, MC Lemardeley and T.Coulhon for their support. They thank the members of MMX group at IPGG, K. Bala, V. Croquette, P.Vitaux, C. Brechot, L.Dehove for discussions and helps, ANR-10-EQPX-0034,IPGG,Institut Pierre Gilles de Gennes pour la microfluidique(2010), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010) |
| المصدر: | https://pasteur.hal.science/pasteur-04670582 ; 2024. |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Objectives Determine the sensitivity and specificity of a Point-Of-Care test (‘COVIDISC’) for SARS-COV2. The novelty of the test is to integrate, on the same (low-cost) compact plastic/paper device, solid phase RNA extraction and RT-LAMP amplification, all reagents being freeze-dried on it. Method Retrospective study with a cohort of 99 patients characterized by real-time RT-PCR. The 37 positive naso-pharyngeal samples cover a broad range of viral loads (from 5 gc /µL to 2 10 6 gc/ µL of sample). Results The COVIDISC found 36 positives (out of 37 by IP4 RT-PCR protocols) and 63 negatives (out of 62 by RT-PCR). Conclusion The sensitivity of the COVIDISC, found in this 99-patient retrospective study, is 97% and the specificity 100%. |
| نوع الوثيقة: | report |
| اللغة: | English |
| Relation: | pasteur-04670582; https://pasteur.hal.science/pasteur-04670582; https://pasteur.hal.science/pasteur-04670582/document; https://pasteur.hal.science/pasteur-04670582/file/2021.10.03.21264480v1.full.pdf; MEDRXIV: 2021.10.03.21264480 |
| DOI: | 10.1101/2021.10.03.21264480 |
| الاتاحة: | https://pasteur.hal.science/pasteur-04670582 https://pasteur.hal.science/pasteur-04670582/document https://pasteur.hal.science/pasteur-04670582/file/2021.10.03.21264480v1.full.pdf https://doi.org/10.1101/2021.10.03.21264480 |
| Rights: | http://hal.archives-ouvertes.fr/licences/copyright/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.7759981D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1101/2021.10.03.21264480 |
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