Academic Journal
Dental pulp mesenchymal stem cell (DPSCs)-derived soluble factors, produced under hypoxic conditions, support angiogenesis via endothelial cell activation and generation of M2-like macrophages
| العنوان: | Dental pulp mesenchymal stem cell (DPSCs)-derived soluble factors, produced under hypoxic conditions, support angiogenesis via endothelial cell activation and generation of M2-like macrophages |
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| المؤلفون: | Barone L., Cucchiara M., Palano M. T., Bassani B., Gallazzi M., Rossi F., Raspanti M., Zecca P. A., De Antoni G., Pagiatakis C., Papait R., Bernardini G., Bruno A., Gornati R. |
| المساهمون: | Barone, L., Cucchiara, M., Palano, M. T., Bassani, B., Gallazzi, M., Rossi, F., Raspanti, M., Zecca, P. A., De Antoni, G., Pagiatakis, C., Papait, R., Bernardini, G., Bruno, A., Gornati, R. |
| سنة النشر: | 2024 |
| المجموعة: | IRInSubria - Institutional Repository Insubria (Università degli Studi dell’Insubria) |
| مصطلحات موضوعية: | Angiogenesi, Cell-free device, Dental pulp stem cell, Macrophage polarization, Mesenchymal stem cell, Secretome, Tissue engineering |
| الوصف: | BackgroundCell therapy has emerged as a revolutionary tool to repair damaged tissues by restoration of an adequate vasculature. Dental Pulp stem cells (DPSC), due to their easy biological access, ex vivo properties, and ability to support angiogenesis have been largely explored in regenerative medicine.MethodsHere, we tested the capability of Dental Pulp Stem Cell-Conditioned medium (DPSC-CM), produced in normoxic (DPSC-CM Normox) or hypoxic (DPSC-CM Hypox) conditions, to support angiogenesis via their soluble factors. CMs were characterized by a secretome protein array, then used for in vivo and in vitro experiments. In in vivo experiments, DPSC-CMs were associated to an Ultimatrix sponge and injected in nude mice. After excision, Ultimatrix were assayed by immunohistochemistry, electron microscopy and flow cytometry, to evaluate the presence of endothelial, stromal, and immune cells.For in vitro procedures, DPSC-CMs were used on human umbilical-vein endothelial cells (HUVECs), to test their effects on cell adhesion, migration, tube formation, and on their capability to recruit human CD14+ monocytes.MethodsHere, we tested the capability of Dental Pulp Stem Cell-Conditioned medium (DPSC-CM), produced in normoxic (DPSC-CM Normox) or hypoxic (DPSC-CM Hypox) conditions, to support angiogenesis via their soluble factors. CMs were characterized by a secretome protein array, then used for in vivo and in vitro experiments. In in vivo experiments, DPSC-CMs were associated to an Ultimatrix sponge and injected in nude mice. After excision, Ultimatrix were assayed by immunohistochemistry, electron microscopy and flow cytometry, to evaluate the presence of endothelial, stromal, and immune cells.For in vitro procedures, DPSC-CMs were used on human umbilical-vein endothelial cells (HUVECs), to test their effects on cell adhesion, migration, tube formation, and on their capability to recruit human CD14+ monocytes.ResultsWe found that DPSC-CM Hypox exert stronger pro-angiogenic activities, compared with DPSC-CM Normox, by ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| Relation: | info:eu-repo/semantics/altIdentifier/pmid/39491013; info:eu-repo/semantics/altIdentifier/wos/WOS:001346673000001; volume:31; issue:1; journal:JOURNAL OF BIOMEDICAL SCIENCE; https://hdl.handle.net/11383/2184654 |
| DOI: | 10.1186/s12929-024-01087-6 |
| الاتاحة: | https://hdl.handle.net/11383/2184654 https://doi.org/10.1186/s12929-024-01087-6 |
| رقم الانضمام: | edsbas.772C515 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12929-024-01087-6 |
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