Academic Journal
Nitrosotalea devaniterrae gen. nov., sp. nov. and Nitrosotalea sinensis sp. nov., two acidophilic ammonia oxidising archaea isolated from acidic soil, and proposal of the new order Nitrosotaleales ord. nov. within the class Nitrososphaeria of the phylum Nitrososphaerota
| العنوان: | Nitrosotalea devaniterrae gen. nov., sp. nov. and Nitrosotalea sinensis sp. nov., two acidophilic ammonia oxidising archaea isolated from acidic soil, and proposal of the new order Nitrosotaleales ord. nov. within the class Nitrososphaeria of the phylum Nitrososphaerota |
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| المؤلفون: | Lehtovirta-Morley, Laura, E, Ge, Chaorong, Ross, Jenna, Yao, Huaiying, Hazard, Christina, Gubry-Rangin, Cécile, Prosser, James, I, Nicol, Graeme, W |
| المساهمون: | Ampère, Département Bioingénierie (BioIng), Ampère (AMPERE), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-École Centrale de Lyon (ECL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| المصدر: | ISSN: 1466-5026. |
| بيانات النشر: | CCSD Microbiology Society |
| سنة النشر: | 2024 |
| مصطلحات موضوعية: | Nitrosotalea, soil, archaeal ammonia oxidiser, nitrification, ammonia oxidation, acidophile AOA, ammonia-oxidising archaea AOB, ammonia-oxidising bacteria amoA, ammonia monooxygenase sub-unit A ANI, average nucleotide identity ATU, allylthiourea DAPI, 4′, 6-diamidino-2-phenylindole DCD, dicyandiamide, ammonia-oxidising archaea, AOB, ammonia-oxidising bacteria, amoA, ammonia monooxygenase sub-unit A, ANI, average nucleotide identity, ATU, allylthiourea, DAPI, 6-diamidino-2-phenylindole, DCD, [SDE]Environmental Sciences |
| الوصف: | International audience ; Two obligately acidophilic, mesophilic and aerobic soil ammonia-oxidising archaea were isolated from a pH 4.5 arable sandy loam (UK) and pH 4.7 acidic sulphate paddy soil (PR China) and designated strains Nd1 T and Nd2 T , respectively. The strains shared more than 99 % 16S rRNA gene sequence identity and their genomes were both less than 2 Mb in length, sharing 79 % average nucleotide identity, 81 % average amino acid identity and a DNA G+C content of approximately 37 mol%. Both strains were chemolithotrophs that fixed carbon dioxide and gained energy by oxidising ammonia to nitrite, with no evidence of mixotrophic growth. Neither strain was capable of using urea as a source of ammonia. Both strains were non-motile in culture, although Nd1 T does possess genes encoding flagella components and therefore may be motile under certain conditions. Cells of Nd1 T were small angular rods 0.5–1 µm in length and grew at pH 4.2–5.6 and at 20–30 °C. Cells of Nd1 T were small angular rods 0.5–1 µm in length and grew at pH 4.0–6.1 and at 20–42 °C. Nd1 T and Nd2 T are distinct with respect to genomic and physiological features and are assigned as the type strains for the species Nitrosotalea devaniterrae sp. nov. (type strain, Nd1 T =NCIMB 15248 T =DSM 110862 T ) and Nitrosotalea sinensis sp. nov. (type strain, Nd2 T =NCIMB 15249 T =DSM 110863 T ), respectively, within the genus Nitrosotalea gen. nov. The family Nitrosotaleaceae fam. nov. and order Nitrosotaleales ord. nov. are also proposed officially. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1099/ijsem.0.006387 |
| الاتاحة: | https://hal.science/hal-04797969 https://hal.science/hal-04797969v1/document https://hal.science/hal-04797969v1/file/Lehtovirta-Morley%20et%20al.%20IJSEM%20Nitrosotalea.pdf https://doi.org/10.1099/ijsem.0.006387 |
| Rights: | http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.770C1A13 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1099/ijsem.0.006387 |
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