Academic Journal
Conformational constraints of cyclopentane peptide nucleic acids facilitate tunable binding to DNA
| العنوان: | Conformational constraints of cyclopentane peptide nucleic acids facilitate tunable binding to DNA |
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| المؤلفون: | Zheng, Hongchao, Botos, Istvan, Clausse, Victor, Nikolayevskiy, Herman, Rastede, Elizabeth E, Fouz, Munira F, Mazur, Sharlyn J, Appella, Daniel H |
| المساهمون: | National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health |
| المصدر: | Nucleic Acids Research ; volume 49, issue 2, page 713-725 ; ISSN 0305-1048 1362-4962 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2021 |
| الوصف: | We report a series of synthetic, nucleic acid mimics with highly customizable thermodynamic binding to DNA. Incorporation of helix-promoting cyclopentanes into peptide nucleic acids (PNAs) increases the melting temperatures (Tm) of PNA+DNA duplexes by approximately +5°C per cyclopentane. Sequential addition of cyclopentanes allows the Tm of PNA + DNA duplexes to be systematically fine-tuned from +5 to +50°C compared with the unmodified PNA. Containing only nine nucleobases and an equal number of cyclopentanes, cpPNA-9 binds to complementary DNA with a Tm around 90°C. Additional experiments reveal that the cpPNA-9 sequence specifically binds to DNA duplexes containing its complementary sequence and functions as a PCR clamp. An X-ray crystal structure of the cpPNA-9–DNA duplex revealed that cyclopentanes likely induce a right-handed helix in the PNA with conformations that promote DNA binding. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/nar/gkaa1249 |
| الاتاحة: | http://dx.doi.org/10.1093/nar/gkaa1249 http://academic.oup.com/nar/article-pdf/49/2/713/36085136/gkaa1249.pdf |
| رقم الانضمام: | edsbas.760815EB |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/nar/gkaa1249 |
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