التفاصيل البيبلوغرافية
| العنوان: |
Evaluation of SARS-CoV-2 ORF7a Deletions from COVID-19-Positive Individuals and Its Impact on Virus Spread in Cell Culture |
| المؤلفون: |
Maria Clara da Costa Simas, Sara Mesquita Costa, Priscila da Silva Figueiredo Celestino Gomes, Nádia Vaez Gonçalves da Cruz, Isadora Alonso Corrêa, Marcos Romário Matos de Souza, Marcos Dornelas-Ribeiro, Tatiana Lucia Santos Nogueira, Caleb Guedes Miranda dos Santos, Luísa Hoffmann, Amilcar Tanuri, Rodrigo Soares de Moura-Neto, Clarissa R. Damaso, Luciana Jesus da Costa, Rosane Silva |
| المصدر: |
Viruses; Volume 15; Issue 3; Pages: 801 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2023 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
SARS-CoV-2, ORF7a, COVID-19, sgRNAs, viral fitness, B.1.1.33 lineage |
| جغرافية الموضوع: |
agris |
| الوصف: |
The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causing the COVID-19 outbreak, posed a primary concern of public health worldwide. The most common changes in SARS-CoV-2 are single nucleotide substitutions, also reported insertions and deletions. This work investigates the presence of SARS-CoV-2 ORF7a deletions identified in COVID-19-positive individuals. Sequencing of SARS-CoV-2 complete genomes showed three different ORF7a size deletions (190-nt, 339-nt and 365-nt). Deletions were confirmed through Sanger sequencing. The ORF7a∆190 was detected in a group of five relatives with mild symptoms of COVID-19, and the ORF7a∆339 and ORF7a∆365 in a couple of co-workers. These deletions did not affect subgenomic RNAs (sgRNA) production downstream of ORF7a. Still, fragments associated with sgRNA of genes upstream of ORF7a showed a decrease in size when corresponding to samples with deletions. In silico analysis suggests that the deletions impair protein proper function; however, isolated viruses with partial deletion of ORF7a can replicate in culture cells similarly to wild-type viruses at 24 hpi, but with less infectious particles after 48 hpi. These findings on deleted ORF7a accessory protein gene, contribute to understanding SARS-CoV-2 phenotypes such as replication, immune evasion and evolutionary fitness as well insights into the role of SARS-CoV-2_ORF7a in the mechanism of virus-host interactions. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| Relation: |
Coronaviruses; https://dx.doi.org/10.3390/v15030801 |
| DOI: |
10.3390/v15030801 |
| الاتاحة: |
https://doi.org/10.3390/v15030801 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| رقم الانضمام: |
edsbas.75A96ABA |
| قاعدة البيانات: |
BASE |