التفاصيل البيبلوغرافية
| العنوان: |
Polymersomes Decorated with the SARS-CoV‑2 Spike Protein Receptor-Binding Domain Elicit Robust Humoral and Cellular Immunity |
| المؤلفون: |
Lisa R. Volpatti (8161437), Rachel P. Wallace (11165105), Shijie Cao (1469452), Michal M. Raczy (11165108), Ruyi Wang (4811667), Laura T. Gray (11165111), Aaron T. Alpar (11165114), Priscilla S. Briquez (11165117), Nikolaos Mitrousis (1495807), Tiffany M. Marchell (11165120), Maria Stella Sasso (11165123), Mindy Nguyen (11165126), Aslan Mansurov (4682680), Erica Budina (8177523), Ani Solanki (11165129), Elyse A. Watkins (10220585), Mathew R. Schnorenberg (11165132), Andrew C. Tremain (11165135), Joseph W. Reda (11165138), Vlad Nicolaescu (11165141), Kevin Furlong (8506896), Steve Dvorkin (11165144), Shann S. Yu (11165147), Balaji Manicassamy (89161), James L. LaBelle (4356589), Matthew V. Tirrell (1715773), Glenn Randall (190008), Marcin Kwissa (11165150), Melody A. Swartz (11165153), Jeffrey A. Hubbell (118232) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Microbiology, Cell Biology, Molecular Biology, Immunology, Cancer, Hematology, Infectious Diseases, Virology, SARS-CoV -2 spike protein receptor-., T cell immunity, COVID -19 pandemic, MPLA, Th 1-type cytokines, neutralizing antibody response, RBD encap, T cell responses, PS, center B cells, RBD surf, lymph nodes, polymersome, nanoparticle-based subunit vaccines |
| الوصف: |
The COVID-19 pandemic underscores the need for rapid, safe, and effective vaccines. In contrast to some traditional vaccines, nanoparticle-based subunit vaccines are particularly efficient in trafficking antigens to lymph nodes, where they induce potent immune cell activation. Here, we developed a strategy to decorate the surface of oxidation-sensitive polymersomes with multiple copies of the SARS-CoV-2 spike protein receptor-binding domain (RBD) to mimic the physical form of a virus particle. We evaluated the vaccination efficacy of these surface-decorated polymersomes (RBD surf ) in mice compared to RBD-encapsulated polymersomes (RBD encap ) and unformulated RBD (RBD free ), using monophosphoryl-lipid-A-encapsulated polymersomes (MPLA PS) as an adjuvant. While all three groups produced high titers of RBD-specific IgG, only RBD surf elicited a neutralizing antibody response to SARS-CoV-2 comparable to that of human convalescent plasma. Moreover, RBD surf was the only group to significantly increase the proportion of RBD-specific germinal center B cells in the vaccination-site draining lymph nodes. Both RBD surf and RBD encap drove similarly robust CD4 + and CD8 + T cell responses that produced multiple Th1-type cytokines. We conclude that a multivalent surface display of spike RBD on polymersomes promotes a potent neutralizing antibody response to SARS-CoV-2, while both antigen formulations promote robust T cell immunity. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| Relation: |
https://figshare.com/articles/journal_contribution/Polymersomes_Decorated_with_the_SARS-CoV_2_Spike_Protein_Receptor-Binding_Domain_Elicit_Robust_Humoral_and_Cellular_Immunity/15028379 |
| DOI: |
10.1021/acscentsci.1c00596.s001 |
| الاتاحة: |
https://doi.org/10.1021/acscentsci.1c00596.s001 |
| Rights: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.75874654 |
| قاعدة البيانات: |
BASE |