Academic Journal
Common Dyes Used to Determine Bacterial Polysaccharides on Agar Are Affected by Medium Acidification
| العنوان: | Common Dyes Used to Determine Bacterial Polysaccharides on Agar Are Affected by Medium Acidification |
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| المؤلفون: | Hawkins, Justin P., Geddes, Barney A., Oresnik, Ivan John |
| بيانات النشر: | University of Toronto |
| سنة النشر: | 2017 |
| المجموعة: | University of Toronto: Research Repository T-Space |
| الوصف: | In this work the effects of pH on bacterial phenotypes when using the bacteriological dyes Aniline blue, Congo red, and Calcofluor white to analyze polysaccharide production. Study of galactose catabolism in Sinorhizobium meliloti led to the isolation of a mutation in dgok1, which was observed to overproduce exopolysaccharides when grown in the presence of galactose. When this strain was spotted onto plates containing Aniline blue, Congo red, or Calcofluor white, the intensity of the associated staining was strikingly different when compared to the Wild-type. Additionally, a Calcofluor dull phenotype was observed, suggesting production of a polysaccharide other than succinoglycan. Investigation of this phenotype revealed that these results were dependent on medium acidification, as buffering at pH 6 had no effect on these phenotypes, while medium buffered at pH 6.5 resulted in a reversal of the phenotypes. Screening for mutants of the dgok1 strain that were negative for the aniline blue phenotype yielded a strain carrying a mutation in a putative transketolase. Consistent with the plate phenotypes, when this mutant was grown in broth cultures it did not acidify its growth medium. This work shows that caution should be exercised in evaluating polysaccharide phenotypes based strictly on the use of dyes. ; The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| تدمد: | 0008-4166 |
| Relation: | http://hdl.handle.net/1807/77180 |
| DOI: | 10.1139/cjm-2016-0743 |
| الاتاحة: | http://hdl.handle.net/1807/77180 http://www.nrcresearchpress.com/doi/abs/10.1139/cjm-2016-0743 |
| رقم الانضمام: | edsbas.7582AE5F |
| قاعدة البيانات: | BASE |
| تدمد: | 00084166 |
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| DOI: | 10.1139/cjm-2016-0743 |