Academic Journal
In situ hybridization for the localization of two pepino mosaic virus isolates in mixed infections
| العنوان: | In situ hybridization for the localization of two pepino mosaic virus isolates in mixed infections |
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| المؤلفون: | Gómez-Aix, Cristina, Alcaide, C., Gómez, Pedro, Aranda, Miguel A., Sánchez-Pina, M. Amelia |
| المساهمون: | Agencia Estatal de Investigación (España), Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España) |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2019 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Nicotiana benthamiana, Viral RNA, In situ hybridization, RNA localization, Mixed infections, PepMV |
| الوصف: | In situ hybridization (ISH) is an informative and relatively accessible technique for the localization of viral genomes in plant tissue and cells. However, simultaneous visualization of related plant viruses in mixed infections may be limited by the nucleotide similarity in the genomes and the single chromogenic detection over the same sample preparation. To address this issue, we used two Pepino mosaic virus isolates and performed ISH over consecutive serial cross-sections of paraffin-embedded leaf samples of single and mixed infected Nicotiana benthamiana plants. Moreover, the probe design was optimized to reduce cross-hybridisation, and co-localization was based on the overlapping of consecutive cross-sections from mixed infected leaves; thus, our results showed that both Pepino mosaic virus isolates co-localized in the same leaf tissue. In turn, both isolates were localized in the cytoplasm of the same cells. These results provide valuable information for studying mixed infections in plants by using a simple ISH procedure that is accessible to any pathology laboratory. ; P.G. and M.A.A. acknowledge funding from MINECO (grants AGL2014-59556-R and PCIN-2017-055, the latter within the ERANET-ARIMNet2 (ref. 302) program), and C.A. was supported by funding from the Ministry of Industry, Economy and Competitiveness (MINECO, Spain) within the PhD programme grant (FPU16/02569). ; Peer reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0166-0934 1879-0984 |
| Relation: | #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-59556-R; PCIN-2017-055/AEI/10.13039/501100011033; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PCIN-2017-055; Postprint; https://doi.org/10.1016/j.jviromet.2019.02.003; Sí; Journal of Virological Methods 267: 42-47 (2019); http://hdl.handle.net/10261/178614; http://dx.doi.org/10.13039/501100003329; http://dx.doi.org/10.13039/501100011033 |
| DOI: | 10.1016/j.jviromet.2019.02.003 |
| DOI: | 10.13039/501100003329 |
| DOI: | 10.13039/501100011033 |
| الاتاحة: | http://hdl.handle.net/10261/178614 https://doi.org/10.1016/j.jviromet.2019.02.003 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/501100011033 |
| Rights: | open |
| رقم الانضمام: | edsbas.754B126E |
| قاعدة البيانات: | BASE |
| تدمد: | 01660934 18790984 |
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| DOI: | 10.1016/j.jviromet.2019.02.003 |